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000049422 0247_ $$2pmid$$apmid:15976924
000049422 0247_ $$2DOI$$a10.1007/s11373-005-6797-z
000049422 0247_ $$2WOS$$aWOS:000230695300002
000049422 037__ $$aPreJuSER-49422
000049422 041__ $$aeng
000049422 082__ $$a610
000049422 084__ $$2WoS$$aMedicine, Research & Experimental
000049422 1001_ $$0P:(DE-HGF)0$$aBriese, L.$$b0
000049422 245__ $$aMapping the binding site of full length HIV-1 Nef on human Lck SH3 by NMR spectroscopy
000049422 260__ $$aLondon$$bBioMed Central$$c2005
000049422 300__ $$a451 - 456
000049422 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article
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000049422 440_0 $$014721$$aJournal of Biomedical Science$$v12$$x1021-7770
000049422 500__ $$aRecord converted from VDB: 12.11.2012
000049422 520__ $$aThe Nef protein of human immunodeficiency virus type 1 (HIV-1) is known to directly bind to the SH3 domain of human lymphocyte specific kinase (Lck) via a proline-rich region located in the amino terminal part of Nef. To address the question whether Nef binding to Lck SH3 involves residues outside the typical poly-proline peptide binding site and whether the Lck unique domain is involved in Nef-Lck interaction, we studied the direct interaction between both molecules using recombinant full-length HIV-1 Nef protein on one side and recombinantly expressed and uniformly 15N-isotope labeled Lck protein comprising unique and SH3 domains on the other side. Applying nuclear magnetic resonance spectroscopy we could show that only residues of Lck SH3, that are typically involved in binding poly-proline peptides, are affected by Nef binding. Further, for the first time we could rule out that residues of Lck unique domain are involved in binding to full length Nef protein. Thus, interactions of Lck unique domain to cellular partners e.g. CD4 or CD8, are not necessarily competitive with Lck binding to HIV-1 Nef.
000049422 536__ $$0G:(DE-Juel1)FUEK255$$2G:(DE-HGF)$$aNeurowissenschaften$$cL01$$x0
000049422 588__ $$aDataset connected to Web of Science, Pubmed
000049422 650_2 $$2MeSH$$aAmino Acid Sequence
000049422 650_2 $$2MeSH$$aBinding Sites
000049422 650_2 $$2MeSH$$aGene Products, nef: chemistry
000049422 650_2 $$2MeSH$$aHIV-1: chemistry
000049422 650_2 $$2MeSH$$aHIV-1: metabolism
000049422 650_2 $$2MeSH$$aHumans
000049422 650_2 $$2MeSH$$aLymphocyte Specific Protein Tyrosine Kinase p56(lck): chemistry
000049422 650_2 $$2MeSH$$aMagnetic Resonance Spectroscopy
000049422 650_2 $$2MeSH$$aModels, Molecular
000049422 650_2 $$2MeSH$$aMolecular Sequence Data
000049422 650_2 $$2MeSH$$aProtein Interaction Mapping
000049422 650_2 $$2MeSH$$aProtein Structure, Tertiary
000049422 650_2 $$2MeSH$$aReceptors, Virus: metabolism
000049422 650_2 $$2MeSH$$aRecombinant Proteins
000049422 650_2 $$2MeSH$$anef Gene Products, Human Immunodeficiency Virus
000049422 650_2 $$2MeSH$$asrc Homology Domains
000049422 650_7 $$00$$2NLM Chemicals$$aGene Products, nef
000049422 650_7 $$00$$2NLM Chemicals$$aReceptors, Virus
000049422 650_7 $$00$$2NLM Chemicals$$aRecombinant Proteins
000049422 650_7 $$00$$2NLM Chemicals$$anef Gene Products, Human Immunodeficiency Virus
000049422 650_7 $$0EC 2.7.10.2$$2NLM Chemicals$$aLymphocyte Specific Protein Tyrosine Kinase p56(lck)
000049422 650_7 $$2WoSType$$aJ
000049422 65320 $$2Author$$achemical shift mapping
000049422 65320 $$2Author$$ahuman immunodeficiency virus
000049422 65320 $$2Author$$aLck
000049422 65320 $$2Author$$aligand binding
000049422 65320 $$2Author$$aNef
000049422 65320 $$2Author$$anuclear magnetic resonance spectroscopy
000049422 65320 $$2Author$$aprotein-protein interaction
000049422 65320 $$2Author$$aprotein structure
000049422 7001_ $$0P:(DE-HGF)0$$aPreusser-Kunze, A.$$b1
000049422 7001_ $$0P:(DE-Juel1)132029$$aWillbold, D.$$b2$$uFZJ
000049422 773__ $$0PERI:(DE-600)1482918-6$$a10.1007/s11373-005-6797-z$$gVol. 12, p. 451 - 456$$p451 - 456$$q12<451 - 456$$tJournal of biomedical science$$v12$$x1021-7770$$y2005
000049422 8567_ $$uhttp://dx.doi.org/10.1007/s11373-005-6797-z
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000049422 9141_ $$y2005
000049422 915__ $$0StatID:(DE-HGF)0010$$aJCR/ISI refereed
000049422 9201_ $$0I:(DE-Juel1)VDB58$$d31.12.2006$$gIBI$$kIBI-2$$lBiologische Strukturforschung$$x0
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000049422 981__ $$aI:(DE-Juel1)ICS-6-20110106