TY  - JOUR
AU  - Briese, L.
AU  - Preusser-Kunze, A.
AU  - Willbold, D.
TI  - Mapping the binding site of full length HIV-1 Nef on human Lck SH3 by NMR spectroscopy
JO  - Journal of biomedical science
VL  - 12
SN  - 1021-7770
CY  - London
PB  - BioMed Central
M1  - PreJuSER-49422
SP  - 451 - 456
PY  - 2005
N1  - Record converted from VDB: 12.11.2012
AB  - The Nef protein of human immunodeficiency virus type 1 (HIV-1) is known to directly bind to the SH3 domain of human lymphocyte specific kinase (Lck) via a proline-rich region located in the amino terminal part of Nef. To address the question whether Nef binding to Lck SH3 involves residues outside the typical poly-proline peptide binding site and whether the Lck unique domain is involved in Nef-Lck interaction, we studied the direct interaction between both molecules using recombinant full-length HIV-1 Nef protein on one side and recombinantly expressed and uniformly 15N-isotope labeled Lck protein comprising unique and SH3 domains on the other side. Applying nuclear magnetic resonance spectroscopy we could show that only residues of Lck SH3, that are typically involved in binding poly-proline peptides, are affected by Nef binding. Further, for the first time we could rule out that residues of Lck unique domain are involved in binding to full length Nef protein. Thus, interactions of Lck unique domain to cellular partners e.g. CD4 or CD8, are not necessarily competitive with Lck binding to HIV-1 Nef.
KW  - Amino Acid Sequence
KW  - Binding Sites
KW  - Gene Products, nef: chemistry
KW  - HIV-1: chemistry
KW  - HIV-1: metabolism
KW  - Humans
KW  - Lymphocyte Specific Protein Tyrosine Kinase p56(lck): chemistry
KW  - Magnetic Resonance Spectroscopy
KW  - Models, Molecular
KW  - Molecular Sequence Data
KW  - Protein Interaction Mapping
KW  - Protein Structure, Tertiary
KW  - Receptors, Virus: metabolism
KW  - Recombinant Proteins
KW  - nef Gene Products, Human Immunodeficiency Virus
KW  - src Homology Domains
KW  - Gene Products, nef (NLM Chemicals)
KW  - Receptors, Virus (NLM Chemicals)
KW  - Recombinant Proteins (NLM Chemicals)
KW  - nef Gene Products, Human Immunodeficiency Virus (NLM Chemicals)
KW  - Lymphocyte Specific Protein Tyrosine Kinase p56(lck) (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:15976924
UR  - <Go to ISI:>//WOS:000230695300002
DO  - DOI:10.1007/s11373-005-6797-z
UR  - https://juser.fz-juelich.de/record/49422
ER  -