000049529 001__ 49529
000049529 005__ 20200423204259.0
000049529 0247_ $$2pmid$$apmid:23115339
000049529 0247_ $$2DOI$$a10.1074/jbcM505012200
000049529 0247_ $$2WOS$$aWOS:000234652000022
000049529 0247_ $$2Handle$$a2128/2646
000049529 037__ $$aPreJuSER-49529
000049529 041__ $$aENG
000049529 082__ $$a570
000049529 084__ $$2WoS$$aBiochemistry & Molecular Biology
000049529 1001_ $$0P:(DE-Juel1)VDB58515$$aBatra-Safferling, R.$$b0$$uFZJ
000049529 245__ $$aGlutamic acid-rich proteins of rod photoreceptors are natively unfolded
000049529 260__ $$aBethesda, Md.$$bSoc.$$c2006
000049529 300__ $$a1449 - 1460
000049529 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article
000049529 3367_ $$2DataCite$$aOutput Types/Journal article
000049529 3367_ $$00$$2EndNote$$aJournal Article
000049529 3367_ $$2BibTeX$$aARTICLE
000049529 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000049529 3367_ $$2DRIVER$$aarticle
000049529 440_0 $$03091$$aJournal of Biological Chemistry$$v281$$x0021-9258$$y3
000049529 500__ $$aRecord converted from VDB: 12.11.2012
000049529 520__ $$aBroadly neutralizing HIV antibodies (bNAbs) can recognize carbohydrate-dependent epitopes on gp120. In contrast to previously characterized glycan-dependent bNAbs that recognize high-mannose N-glycans, PGT121 binds complex-type N-glycans in glycan microarrays. We isolated the B-cell clone encoding PGT121, which segregates into PGT121-like and 10-1074-like groups distinguished by sequence, binding affinity, carbohydrate recognition, and neutralizing activity. Group 10-1074 exhibits remarkable potency and breadth but no detectable binding to protein-free glycans. Crystal structures of unliganded PGT121, 10-1074, and their likely germ-line precursor reveal that differential carbohydrate recognition maps to a cleft between complementarity determining region (CDR)H2 and CDRH3. This cleft was occupied by a complex-type N-glycan in a "liganded" PGT121 structure. Swapping glycan contact residues between PGT121 and 10-1074 confirmed their importance for neutralization. Although PGT121 binds complex-type N-glycans, PGT121 recognized high-mannose-only HIV envelopes in isolation and on virions. As HIV envelopes exhibit varying proportions of high-mannose- and complex-type N-glycans, these results suggest promiscuous carbohydrate interactions, an advantageous adaptation ensuring neutralization of all viruses within a given strain.
000049529 536__ $$0G:(DE-Juel1)FUEK409$$2G:(DE-HGF)$$aFunktion und Dysfunktion des Nervensystems$$cP33$$x0
000049529 588__ $$aDataset connected to Web of Science, Pubmed
000049529 650_7 $$2WoSType$$aJ
000049529 7001_ $$0P:(DE-Juel1)VDB58516$$aAbarca-Heidemann, K.$$b1$$uFZJ
000049529 7001_ $$0P:(DE-Juel1)VDB31218$$aKörschen, H. G.$$b2$$uFZJ
000049529 7001_ $$0P:(DE-HGF)0$$aTziatzios, C.$$b3
000049529 7001_ $$0P:(DE-Juel1)VDB21601$$aStoldt, M.$$b4$$uFZJ
000049529 7001_ $$0P:(DE-Juel1)VDB44596$$aBudyak, I.$$b5$$uFZJ
000049529 7001_ $$0P:(DE-Juel1)132029$$aWillbold, D.$$b6$$uFZJ
000049529 7001_ $$0P:(DE-HGF)0$$aSchwalbe, H.$$b7
000049529 7001_ $$0P:(DE-Juel1)VDB44599$$aKlein-Seetharaman, J.$$b8$$uFZJ
000049529 7001_ $$0P:(DE-Juel1)VDB728$$aKaupp, U. B.$$b9$$uFZJ
000049529 773__ $$0PERI:(DE-600)1474604-9$$a10.1074/jbcM505012200$$gVol. 281, p. 1449 - 1460$$p1449 - 1460$$q281<1449 - 1460$$tThe @journal of biological chemistry$$v281$$x0021-9258$$y2006
000049529 8567_ $$uhttp://hdl.handle.net/2128/2646$$uhttp://dx.doi.org/10.1074/jbcM505012200
000049529 8564_ $$uhttps://juser.fz-juelich.de/record/49529/files/77520.pdf$$yOpenAccess
000049529 8564_ $$uhttps://juser.fz-juelich.de/record/49529/files/77520.jpg?subformat=icon-1440$$xicon-1440$$yOpenAccess
000049529 8564_ $$uhttps://juser.fz-juelich.de/record/49529/files/77520.jpg?subformat=icon-180$$xicon-180$$yOpenAccess
000049529 8564_ $$uhttps://juser.fz-juelich.de/record/49529/files/77520.jpg?subformat=icon-640$$xicon-640$$yOpenAccess
000049529 909CO $$ooai:juser.fz-juelich.de:49529$$pdnbdelivery$$pVDB$$pdriver$$popen_access$$popenaire
000049529 9131_ $$0G:(DE-Juel1)FUEK409$$bGesundheit$$kP33$$lFunktion und Dysfunktion des Nervensystems$$vFunktion und Dysfunktion des Nervensystems$$x0
000049529 9141_ $$y2006
000049529 915__ $$0StatID:(DE-HGF)0010$$aJCR/ISI refereed
000049529 915__ $$0StatID:(DE-HGF)0510$$2StatID$$aOpenAccess
000049529 9201_ $$0I:(DE-Juel1)VDB57$$d31.12.2006$$gIBI$$kIBI-1$$lZelluläre Signalverarbeitung$$x0
000049529 9201_ $$0I:(DE-Juel1)VDB58$$d31.12.2006$$gIBI$$kIBI-2$$lBiologische Strukturforschung$$x1
000049529 970__ $$aVDB:(DE-Juel1)77520
000049529 9801_ $$aFullTexts
000049529 980__ $$aVDB
000049529 980__ $$aJUWEL
000049529 980__ $$aConvertedRecord
000049529 980__ $$ajournal
000049529 980__ $$aI:(DE-Juel1)ICS-4-20110106
000049529 980__ $$aI:(DE-Juel1)ISB-2-20090406
000049529 980__ $$aUNRESTRICTED
000049529 980__ $$aI:(DE-Juel1)ICS-6-20110106
000049529 980__ $$aFullTexts
000049529 981__ $$aI:(DE-Juel1)IBI-1-20200312
000049529 981__ $$aI:(DE-Juel1)IBI-7-20200312
000049529 981__ $$aI:(DE-Juel1)ICS-4-20110106
000049529 981__ $$aI:(DE-Juel1)ISB-2-20090406
000049529 981__ $$aI:(DE-Juel1)ICS-6-20110106