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000049745 0247_ $$2DOI$$a10.1002/bit.20533
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000049745 084__ $$2WoS$$aBiotechnology & Applied Microbiology
000049745 1001_ $$0P:(DE-HGF)0$$aAnastassiadis, A.$$b0
000049745 245__ $$aProcess Optimization of Continuous Gluconic Acid Fermentation by Isolated Yeast-Like Strains of Aureobasidium pullulans
000049745 260__ $$aNew York, NY [u.a.]$$bWiley$$c2005
000049745 300__ $$a494 - 501
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000049745 440_0 $$0900$$aBiotechnology and Bioengineering$$v91$$x0006-3592$$y4
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000049745 520__ $$aThis study was focused on the optimization of a new fermentation process for continuous gluconic acid production by the isolated yeast-like strain Aureobasidium pullulans DSM 7085 (isolate 70). Operational fermentation parameters were optimized in chemostat cultures, using a defined glucose medium. Different optima were found for growth and gluconic acid production for each set of operation parameters. Highest productivity was recorded at pH values between 6.5 and 7.0 and temperatures between 29 and 31 degrees C. A gluconic acid concentration higher than 230 g/L was continuously produced at residence times of 12 h. A steady state extracellular gluconic acid concentration of 234 g/L was measured at pH 6.5. 122% air saturation yielded the highest volumetric productivity and product concentration. The biomass-specific productivity increased steadily upon raising air saturation. An intracellular gluconic acid concentration of about 159 g/L (0.83 mol) was determined at 31 degrees C. This is to be compared with an extracellular concentration of 223 g/L (1.16 mol), which indicates the possible existence of an active transport system for gluconic acid secretion, or the presence of extracellular glucose oxidizing enzymes. The new process provides significant advantages over the traditional discontinuous fungi operations. The process control becomes easier, thus offering stable product quality and quantity.
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000049745 650_2 $$2MeSH$$aAscomycota: enzymology
000049745 650_2 $$2MeSH$$aAscomycota: physiology
000049745 650_2 $$2MeSH$$aBioreactors
000049745 650_2 $$2MeSH$$aFermentation
000049745 650_2 $$2MeSH$$aGluconates: metabolism
000049745 650_2 $$2MeSH$$aHot Temperature
000049745 650_2 $$2MeSH$$aHumidity
000049745 650_2 $$2MeSH$$aHydrogen-Ion Concentration
000049745 650_2 $$2MeSH$$aMicrobiological Techniques: methods
000049745 650_2 $$2MeSH$$aOxidation-Reduction
000049745 650_2 $$2MeSH$$aOxygen: metabolism
000049745 650_7 $$00$$2NLM Chemicals$$aGluconates
000049745 650_7 $$0526-95-4$$2NLM Chemicals$$agluconic acid
000049745 650_7 $$07782-44-7$$2NLM Chemicals$$aOxygen
000049745 650_7 $$2WoSType$$aJ
000049745 65320 $$2Author$$acontinuous gluconic acid fermentation
000049745 65320 $$2Author$$acontinuous fermentation
000049745 65320 $$2Author$$agluconic acid
000049745 65320 $$2Author$$aAureobasidium pullulans
000049745 65320 $$2Author$$aprocess development
000049745 65320 $$2Author$$aprocess optimization
000049745 7001_ $$0P:(DE-HGF)0$$aAivasidis, A.$$b1
000049745 7001_ $$0P:(DE-Juel1)129071$$aWandrey, C.$$b2$$uFZJ
000049745 7001_ $$0P:(DE-HGF)0$$aRehm, H.-J.$$b3
000049745 773__ $$0PERI:(DE-600)1480809-2$$a10.1002/bit.20533$$gVol. 91, p. 494 - 501$$p494 - 501$$q91<494 - 501$$tBiotechnology & bioengineering$$v91$$x0006-3592$$y2005
000049745 8567_ $$uhttp://dx.doi.org/10.1002/bit.20533
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