TY  - JOUR
AU  - Anastassiadis, A.
AU  - Wandrey, C.
AU  - Rehm, H.-J.
TI  - Continuous citric acid fermentation by Candida oleophila under nitrogen limitation at constant C/N ratio
JO  - World journal of microbiology and biotechnology
VL  - 21
SN  - 0959-3993
CY  - Dordrecht [u.a.]
PB  - Springer Science + Business Media B.V
M1  - PreJuSER-49746
SP  - 695 - 705
PY  - 2005
N1  - Record converted from VDB: 12.11.2012
AB  - The central aspect of this work was to investigate the influence of nitrogen feed rate at constant C/N ratio on continuous citric acid fermentation by Candida oleophila ATCC 20177. Medium ammonia nitrogen and glucose concentrations influenced growth and production. Space-time yield (STY) meaning volumetric productivity, biomass specific productivity (BSP), product concentration, product selectivity and citrate/isocitrate ratio increased with increasing residence time (RT). BSP increased in an exponential mode lowering nitrogen feed rates. Highest BSP for citric acid of 0.13 g/(g h) was achieved at lowest NH4Cl concentration of 1.5 g/l and highest STY (1.2 g/l h) with 3 g NH4Cl/l at a RT of 25 h. Citric acid 74.2 g/l were produced at 58 h RT and 6 g NH4Cl/l. Glucose uptake rate seems to be strictly controlled by the growth rate of the yeast cells. Optimum nitrogen concentration and adapted C/N ratio are essential for successful continuous citric acid fermentation. The biomass-specific nitrogen feed rate is the most important factor influencing continuous citric acid production by yeasts. Numerous chemostat experiments showed the feasibility of continuous citrate production by yeasts.
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000232158900014
DO  - DOI:10.1007/s11274-004-3850-4
UR  - https://juser.fz-juelich.de/record/49746
ER  -