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@ARTICLE{Anastassiadis:49746,
author = {Anastassiadis, A. and Wandrey, C. and Rehm, H.-J.},
title = {{C}ontinuous citric acid fermentation by {C}andida
oleophila under nitrogen limitation at constant {C}/{N}
ratio},
journal = {World journal of microbiology and biotechnology},
volume = {21},
issn = {0959-3993},
address = {Dordrecht [u.a.]},
publisher = {Springer Science + Business Media B.V},
reportid = {PreJuSER-49746},
pages = {695 - 705},
year = {2005},
note = {Record converted from VDB: 12.11.2012},
abstract = {The central aspect of this work was to investigate the
influence of nitrogen feed rate at constant C/N ratio on
continuous citric acid fermentation by Candida oleophila
ATCC 20177. Medium ammonia nitrogen and glucose
concentrations influenced growth and production. Space-time
yield (STY) meaning volumetric productivity, biomass
specific productivity (BSP), product concentration, product
selectivity and citrate/isocitrate ratio increased with
increasing residence time (RT). BSP increased in an
exponential mode lowering nitrogen feed rates. Highest BSP
for citric acid of 0.13 g/(g h) was achieved at lowest NH4Cl
concentration of 1.5 g/l and highest STY (1.2 g/l h) with 3
g NH4Cl/l at a RT of 25 h. Citric acid 74.2 g/l were
produced at 58 h RT and 6 g NH4Cl/l. Glucose uptake rate
seems to be strictly controlled by the growth rate of the
yeast cells. Optimum nitrogen concentration and adapted C/N
ratio are essential for successful continuous citric acid
fermentation. The biomass-specific nitrogen feed rate is the
most important factor influencing continuous citric acid
production by yeasts. Numerous chemostat experiments showed
the feasibility of continuous citrate production by yeasts.},
keywords = {J (WoSType)},
cin = {IBT-2},
ddc = {570},
cid = {I:(DE-Juel1)VDB56},
pnm = {Biotechnologie},
pid = {G:(DE-Juel1)FUEK256},
shelfmark = {Biotechnology $\&$ Applied Microbiology},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000232158900014},
doi = {10.1007/s11274-004-3850-4},
url = {https://juser.fz-juelich.de/record/49746},
}