001     49746
005     20180211185456.0
024 7 _ |2 DOI
|a 10.1007/s11274-004-3850-4
024 7 _ |2 WOS
|a WOS:000232158900014
037 _ _ |a PreJuSER-49746
041 _ _ |a eng
082 _ _ |a 570
084 _ _ |2 WoS
|a Biotechnology & Applied Microbiology
100 1 _ |a Anastassiadis, A.
|b 0
|0 P:(DE-HGF)0
245 _ _ |a Continuous citric acid fermentation by Candida oleophila under nitrogen limitation at constant C/N ratio
260 _ _ |a Dordrecht [u.a.]
|b Springer Science + Business Media B.V
|c 2005
300 _ _ |a 695 - 705
336 7 _ |a Journal Article
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336 7 _ |a ARTICLE
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336 7 _ |a article
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440 _ 0 |a World Journal of Microbiology & Biotechnology
|x 0959-3993
|0 14825
|v 21
500 _ _ |a Record converted from VDB: 12.11.2012
520 _ _ |a The central aspect of this work was to investigate the influence of nitrogen feed rate at constant C/N ratio on continuous citric acid fermentation by Candida oleophila ATCC 20177. Medium ammonia nitrogen and glucose concentrations influenced growth and production. Space-time yield (STY) meaning volumetric productivity, biomass specific productivity (BSP), product concentration, product selectivity and citrate/isocitrate ratio increased with increasing residence time (RT). BSP increased in an exponential mode lowering nitrogen feed rates. Highest BSP for citric acid of 0.13 g/(g h) was achieved at lowest NH4Cl concentration of 1.5 g/l and highest STY (1.2 g/l h) with 3 g NH4Cl/l at a RT of 25 h. Citric acid 74.2 g/l were produced at 58 h RT and 6 g NH4Cl/l. Glucose uptake rate seems to be strictly controlled by the growth rate of the yeast cells. Optimum nitrogen concentration and adapted C/N ratio are essential for successful continuous citric acid fermentation. The biomass-specific nitrogen feed rate is the most important factor influencing continuous citric acid production by yeasts. Numerous chemostat experiments showed the feasibility of continuous citrate production by yeasts.
536 _ _ |a Biotechnologie
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588 _ _ |a Dataset connected to Web of Science
650 _ 7 |a J
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653 2 0 |2 Author
|a Candida oleophila
653 2 0 |2 Author
|a citric acid production
653 2 0 |2 Author
|a continuous citric acid fermentation
653 2 0 |2 Author
|a nitrogen influence
653 2 0 |2 Author
|a specific nitrogen feed rate
653 2 0 |2 Author
|a specific active citrate transport system
700 1 _ |a Wandrey, C.
|b 1
|u FZJ
|0 P:(DE-Juel1)129071
700 1 _ |a Rehm, H.-J.
|b 2
|0 P:(DE-HGF)0
773 _ _ |a 10.1007/s11274-004-3850-4
|g Vol. 21, p. 695 - 705
|p 695 - 705
|q 21<695 - 705
|0 PERI:(DE-600)1499109-3
|t World journal of microbiology and biotechnology
|v 21
|y 2005
|x 0959-3993
856 7 _ |u http://dx.doi.org/10.1007/s11274-004-3850-4
909 C O |o oai:juser.fz-juelich.de:49746
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914 1 _ |y 2005
915 _ _ |0 StatID:(DE-HGF)0010
|a JCR/ISI refereed
920 1 _ |k IBT-2
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980 _ _ |a UNRESTRICTED
981 _ _ |a I:(DE-Juel1)IBG-1-20101118


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