TY  - JOUR
AU  - Matsubara, S.
AU  - Hurry, V.
AU  - Druart, N.
AU  - Benedict, C.
AU  - Janzik, I.
AU  - Chavarria-Krauser, A.
AU  - Walter, A.
AU  - Schurr, U.
TI  - Nocturnal changes in leaf growth of Populus deltoides are controlled by cytoplasmic growth
JO  - Planta
VL  - 223
SN  - 0032-0935
CY  - Berlin
PB  - Springer
M1  - PreJuSER-49905
SP  - 1315 - 1328
PY  - 2006
N1  - Record converted from VDB: 12.11.2012
AB  - Growing leaves do not expand at a constant rate but exhibit pronounced diel growth rhythms. However, the mechanisms giving rise to distinct diel growth dynamics in different species are still largely unknown. As a first step towards identifying genes controlling rate and timing of leaf growth, we analysed the transcriptomes of rapidly expanding and fully expanded leaves of Populus deltoides Bartr. ex. Marsh at points of high and low expansion at night. Tissues with well defined temporal growth rates were harvested using an online growth-monitoring system based on a digital image sequence processing method developed for quantitative mapping of dicot leaf growth. Unlike plants studied previously, leaf growth in P. deltoides was characterised by lack of a base-tip gradient across the lamina, and by maximal and minimal growth at dusk and dawn, respectively. Microarray analysis revealed that the nocturnal decline in growth coincided with a concerted down-regulation of ribosomal protein genes, indicating deceleration of cytoplasmic growth. In a subsequent time-course experiment, Northern blotting and real-time RT-PCR confirmed that the ribosomal protein gene RPL12 and a cell-cycle gene H2B were down-regulated after midnight following a decrease in cellular carbohydrate concentrations. Thus, we propose that the spatio-temporal growth pattern in leaves of P. deltoides primarily arises from cytoplasmic growth whose activity increases from afternoon to midnight and thereafter decreases in this species.
KW  - Carbohydrate Metabolism
KW  - Circadian Rhythm
KW  - Cluster Analysis
KW  - Cytoplasm: physiology
KW  - Darkness
KW  - Gene Expression Profiling
KW  - Gene Expression Regulation, Plant
KW  - Genes, Plant
KW  - Histones: genetics
KW  - Histones: metabolism
KW  - Oligonucleotide Array Sequence Analysis
KW  - Plant Leaves: cytology
KW  - Plant Leaves: growth & development
KW  - Plant Proteins: genetics
KW  - Plant Proteins: metabolism
KW  - Populus: cytology
KW  - Populus: growth & development
KW  - Ribosomal Proteins: genetics
KW  - Ribosomal Proteins: metabolism
KW  - Histones (NLM Chemicals)
KW  - Plant Proteins (NLM Chemicals)
KW  - Ribosomal Proteins (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:16333638
UR  - <Go to ISI:>//WOS:000237335300020
DO  - DOI:10.1007/s00425-005-0181-0
UR  - https://juser.fz-juelich.de/record/49905
ER  -