%0 Journal Article
%A Liebich, J.
%A Schadt, C. W.
%A Chong, S. C.
%A He, Z.
%A Rhee, S.-K.
%A Zhou, J.-H.
%T Improvement of Oligonucleotide Probe Design Criteria for Functional Gene Microarrays in Environmental applications
%J Applied and environmental microbiology
%V 72
%@ 0099-2240
%C Washington, DC [u.a.]
%I Soc.
%M PreJuSER-50473
%P 1688 - 1691
%D 2006
%Z Record converted from VDB: 12.11.2012
%X To optimize oligonucleotide probe design criteria, PCR products with different similarities to probes were hybridized to a functional gene microarray designed to detect homologous genes from different organisms. In contrast to more restrictive probe designs based on a single criterion, simultaneous consideration of the percent similarity (< or =90%), the length of identical sequence stretches (< or =20 bases), and the binding free energy (> or =-35 kcal mol(-1)) was found to be predictive of probe specificity.
%K Base Sequence
%K Drug Design
%K Environmental Microbiology
%K Nucleic Acid Hybridization
%K Oligonucleotide Array Sequence Analysis: methods
%K Oligonucleotide Array Sequence Analysis: statistics & numerical data
%K Oligonucleotide Probes: chemistry
%K Oligonucleotide Probes: genetics
%K Polymerase Chain Reaction
%K Thermodynamics
%K Oligonucleotide Probes (NLM Chemicals)
%K J (WoSType)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:16461729
%2 pmc:PMC1392891
%U <Go to ISI:>//WOS:000235353100092
%R 10.1128/AEM.72.2.1688-1691.2006
%U https://juser.fz-juelich.de/record/50473