TY  - JOUR
AU  - Liebich, J.
AU  - Schadt, C. W.
AU  - Chong, S. C.
AU  - He, Z.
AU  - Rhee, S.-K.
AU  - Zhou, J.-H.
TI  - Improvement of Oligonucleotide Probe Design Criteria for Functional Gene Microarrays in Environmental applications
JO  - Applied and environmental microbiology
VL  - 72
SN  - 0099-2240
CY  - Washington, DC [u.a.]
PB  - Soc.
M1  - PreJuSER-50473
SP  - 1688 - 1691
PY  - 2006
N1  - Record converted from VDB: 12.11.2012
AB  - To optimize oligonucleotide probe design criteria, PCR products with different similarities to probes were hybridized to a functional gene microarray designed to detect homologous genes from different organisms. In contrast to more restrictive probe designs based on a single criterion, simultaneous consideration of the percent similarity (< or =90%), the length of identical sequence stretches (< or =20 bases), and the binding free energy (> or =-35 kcal mol(-1)) was found to be predictive of probe specificity.
KW  - Base Sequence
KW  - Drug Design
KW  - Environmental Microbiology
KW  - Nucleic Acid Hybridization
KW  - Oligonucleotide Array Sequence Analysis: methods
KW  - Oligonucleotide Array Sequence Analysis: statistics & numerical data
KW  - Oligonucleotide Probes: chemistry
KW  - Oligonucleotide Probes: genetics
KW  - Polymerase Chain Reaction
KW  - Thermodynamics
KW  - Oligonucleotide Probes (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:16461729
C2  - pmc:PMC1392891
UR  - <Go to ISI:>//WOS:000235353100092
DO  - DOI:10.1128/AEM.72.2.1688-1691.2006
UR  - https://juser.fz-juelich.de/record/50473
ER  -