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@ARTICLE{Liebich:50473,
      author       = {Liebich, J. and Schadt, C. W. and Chong, S. C. and He, Z.
                      and Rhee, S.-K. and Zhou, J.-H.},
      title        = {{I}mprovement of {O}ligonucleotide {P}robe {D}esign
                      {C}riteria for {F}unctional {G}ene {M}icroarrays in
                      {E}nvironmental applications},
      journal      = {Applied and environmental microbiology},
      volume       = {72},
      issn         = {0099-2240},
      address      = {Washington, DC [u.a.]},
      publisher    = {Soc.},
      reportid     = {PreJuSER-50473},
      pages        = {1688 - 1691},
      year         = {2006},
      note         = {Record converted from VDB: 12.11.2012},
      abstract     = {To optimize oligonucleotide probe design criteria, PCR
                      products with different similarities to probes were
                      hybridized to a functional gene microarray designed to
                      detect homologous genes from different organisms. In
                      contrast to more restrictive probe designs based on a single
                      criterion, simultaneous consideration of the percent
                      similarity (< or $=90\%),$ the length of identical sequence
                      stretches (< or =20 bases), and the binding free energy (>
                      or =-35 kcal mol(-1)) was found to be predictive of probe
                      specificity.},
      keywords     = {Base Sequence / Drug Design / Environmental Microbiology /
                      Nucleic Acid Hybridization / Oligonucleotide Array Sequence
                      Analysis: methods / Oligonucleotide Array Sequence Analysis:
                      statistics $\&$ numerical data / Oligonucleotide Probes:
                      chemistry / Oligonucleotide Probes: genetics / Polymerase
                      Chain Reaction / Thermodynamics / Oligonucleotide Probes
                      (NLM Chemicals) / J (WoSType)},
      cin          = {ICG-IV},
      ddc          = {570},
      cid          = {I:(DE-Juel1)VDB50},
      pnm          = {Terrestrische Umwelt},
      pid          = {G:(DE-Juel1)FUEK407},
      shelfmark    = {Biotechnology $\&$ Applied Microbiology / Microbiology},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:16461729},
      pmc          = {pmc:PMC1392891},
      UT           = {WOS:000235353100092},
      doi          = {10.1128/AEM.72.2.1688-1691.2006},
      url          = {https://juser.fz-juelich.de/record/50473},
}