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024 7 _ |a pmid:16461729
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024 7 _ |a pmc:PMC1392891
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024 7 _ |a 10.1128/AEM.72.2.1688-1691.2006
|2 DOI
024 7 _ |a WOS:000235353100092
|2 WOS
024 7 _ |a 2128/796
|2 Handle
024 7 _ |a altmetric:21814924
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037 _ _ |a PreJuSER-50473
041 _ _ |a eng
082 _ _ |a 570
084 _ _ |2 WoS
|a Biotechnology & Applied Microbiology
084 _ _ |2 WoS
|a Microbiology
100 1 _ |a Liebich, J.
|b 0
|u FZJ
|0 P:(DE-Juel1)VDB940
245 _ _ |a Improvement of Oligonucleotide Probe Design Criteria for Functional Gene Microarrays in Environmental applications
260 _ _ |a Washington, DC [u.a.]
|b Soc.
|c 2006
300 _ _ |a 1688 - 1691
336 7 _ |a Journal Article
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336 7 _ |a Output Types/Journal article
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336 7 _ |a Journal Article
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336 7 _ |a ARTICLE
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336 7 _ |a JOURNAL_ARTICLE
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336 7 _ |a article
|2 DRIVER
440 _ 0 |a Applied and Environmental Microbiology
|x 0099-2240
|0 8561
|y 2
|v 72
500 _ _ |a Record converted from VDB: 12.11.2012
520 _ _ |a To optimize oligonucleotide probe design criteria, PCR products with different similarities to probes were hybridized to a functional gene microarray designed to detect homologous genes from different organisms. In contrast to more restrictive probe designs based on a single criterion, simultaneous consideration of the percent similarity (< or =90%), the length of identical sequence stretches (< or =20 bases), and the binding free energy (> or =-35 kcal mol(-1)) was found to be predictive of probe specificity.
536 _ _ |a Terrestrische Umwelt
|c P24
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588 _ _ |a Dataset connected to Web of Science, Pubmed
650 _ 2 |2 MeSH
|a Base Sequence
650 _ 2 |2 MeSH
|a Drug Design
650 _ 2 |2 MeSH
|a Environmental Microbiology
650 _ 2 |2 MeSH
|a Nucleic Acid Hybridization
650 _ 2 |2 MeSH
|a Oligonucleotide Array Sequence Analysis: methods
650 _ 2 |2 MeSH
|a Oligonucleotide Array Sequence Analysis: statistics & numerical data
650 _ 2 |2 MeSH
|a Oligonucleotide Probes: chemistry
650 _ 2 |2 MeSH
|a Oligonucleotide Probes: genetics
650 _ 2 |2 MeSH
|a Polymerase Chain Reaction
650 _ 2 |2 MeSH
|a Thermodynamics
650 _ 7 |0 0
|2 NLM Chemicals
|a Oligonucleotide Probes
650 _ 7 |a J
|2 WoSType
700 1 _ |a Schadt, C. W.
|b 1
|0 P:(DE-HGF)0
700 1 _ |a Chong, S. C.
|b 2
|0 P:(DE-HGF)0
700 1 _ |a He, Z.
|b 3
|0 P:(DE-HGF)0
700 1 _ |a Rhee, S.-K.
|b 4
|0 P:(DE-HGF)0
700 1 _ |a Zhou, J.-H.
|b 5
|0 P:(DE-HGF)0
773 _ _ |a 10.1128/AEM.72.2.1688-1691.2006
|g Vol. 72, p. 1688 - 1691
|p 1688 - 1691
|q 72<1688 - 1691
|0 PERI:(DE-600)1478346-0
|t Applied and environmental microbiology
|v 72
|y 2006
|x 0099-2240
856 7 _ |2 Pubmed Central
|u http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1392891
856 4 _ |u https://juser.fz-juelich.de/record/50473/files/78793.pdf
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914 1 _ |y 2006
915 _ _ |0 StatID:(DE-HGF)0010
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