TY  - JOUR
AU  - Csiszar, A.
AU  - Koglin, E.
AU  - Meier, R.
AU  - Klumpp, E.
TI  - The phase transition behavior of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) model membrane influenced by 2,4-dichlorophenol – an FT-Raman Spectroscopy Study
JO  - Chemistry and physics of lipids
VL  - 139
SN  - 0009-3084
CY  - Amsterdam [u.a.]
PB  - Elsevier Science
M1  - PreJuSER-50645
SP  - 115 - 124
PY  - 2006
N1  - Record converted from VDB: 12.11.2012
AB  - The effect of 2,4-dichlorophenol (DCP) on the structures and phase transitions of fully hydrated 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) liposomes was studied using FT-Raman spectroscopy. Whereas the Raman frequency shifts of the most frequently investigated bands of C-C and C-H stretching regions only indicate the main phase transition (P(beta')-L(alpha)) of the pure DPPC/water system, the Raman shift of C-H scissoring vibration at 1440 cm(-1) was found to be able to reveal the pretransition (L(beta')-P(beta')) as well. Analyzing the spectral parameters of the trans band at 1128 cm(-1), which does not overlap with DCP vibrational modes, a continuous decrease of trans conformations was found with increasing DCP concentration at 26 degrees C accompanying the phase transitions L(beta')-P(beta') and P(beta')-L(alpha). The intensity ratio of the symmetrical and asymmetrical methylene stretching bands (at 2850 cm(-1) and 2880 cm(-1)), defined as the disorder parameter by Levin [Levin, I.W., 1985. Two types of hydrocarbon chain interdigitation in sphingomielin bilayers. Biochemistry 24, 6282-6286], indicated that in the interdigitated phase (L(I)) the order is markedly high and comparable with that of L(beta). Both the phase transition P(beta')-L(alpha) in the DCP/DPPC molar ratio range of 10/100-50/100 and the phase transition L(I)-L(alpha) led to a significant increase of disordered chains and the presence of DCP molecules induced a more disordered chain region than that observed in the L(alpha) phase of DPPC. Nevertheless, it was found that the L(alpha) phase with DCP contains approximately the same amount of trans conformers than that without DCP.
KW  - 1,2-Dipalmitoylphosphatidylcholine: chemistry
KW  - Chemistry, Physical
KW  - Chlorophenols: chemistry
KW  - Membranes, Artificial
KW  - Phase Transition
KW  - Physicochemical Phenomena
KW  - Sensitivity and Specificity
KW  - Spectrum Analysis, Raman: methods
KW  - Temperature
KW  - Chlorophenols (NLM Chemicals)
KW  - Membranes, Artificial (NLM Chemicals)
KW  - 2,4-dichlorophenol (NLM Chemicals)
KW  - 1,2-Dipalmitoylphosphatidylcholine (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:16413520
UR  - <Go to ISI:>//WOS:000235590400004
DO  - DOI:10.1016/j.chemphyslip.2005.11.005
UR  - https://juser.fz-juelich.de/record/50645
ER  -