TY  - JOUR
AU  - Gögelein, C.
AU  - Nägele, G.
AU  - Tuinier, R.
AU  - Gibaud, T.
AU  - Stradner, A.
AU  - Schurtenberger, P.
TI  - A simple patchy colloid model for the phase behavior of lysozyme dispersions
JO  - The journal of chemical physics
VL  - 129
SN  - 0021-9606
CY  - Melville, NY
PB  - American Institute of Physics
M1  - PreJuSER-508
SP  - 085102
PY  - 2008
N1  - This project has been partly supported by the European Commission under the 6<SUP>th</SUP> Framework Program through integrating and strengthening the European Research Area. Contract No. SoftComp, NoE/NMP3-CT-2004-502235.
AB  - We propose a minimal model for spherical proteins with aeolotopic pair interactions to describe the equilibrium phase behavior of lysozyme. The repulsive screened Coulomb interactions between the particles are taken into account assuming that the net charges are smeared out homogeneously over the spherical protein surfaces. We incorporate attractive surface patches, with the interactions between patches on different spheres modeled by an attractive Yukawa potential. The parameters entering the attractive Yukawa potential part are determined using information on the experimentally accessed gas-liquid-like critical point. The Helmholtz free energy of the fluid and solid phases is calculated using second-order thermodynamic perturbation theory. Our predictions for the solubility curve are in fair agreement with experimental data. In addition, we present new experimental data for the gas-liquid coexistence curves at various salt concentrations and compare these with our model calculations. In agreement with earlier findings, we observe that the strength and the range of the attractive potential part only weakly depend on the salt content.
KW  - Algorithms
KW  - Animals
KW  - Biophysics: methods
KW  - Chemistry, Physical: methods
KW  - Chickens
KW  - Colloids: chemistry
KW  - Egg Proteins: chemistry
KW  - Hydrogen-Ion Concentration
KW  - Models, Statistical
KW  - Models, Theoretical
KW  - Muramidase: chemistry
KW  - Solubility
KW  - Solvents: chemistry
KW  - Temperature
KW  - Thermodynamics
KW  - Colloids (NLM Chemicals)
KW  - Egg Proteins (NLM Chemicals)
KW  - Solvents (NLM Chemicals)
KW  - Muramidase (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:19044852
UR  - <Go to ISI:>//WOS:000259008900044
DO  - DOI:10.1063/1.2951987
UR  - https://juser.fz-juelich.de/record/508
ER  -