TY  - JOUR
AU  - Wennerhold, J.
AU  - Bott, M.
TI  - The DtxR Regulon of Corynebacterium glutamicum
JO  - Journal of bacteriology
VL  - 188
SN  - 0021-9193
CY  - Washington, DC
PB  - Soc.
M1  - PreJuSER-50945
SP  - 2907 - 2918
PY  - 2006
N1  - Record converted from VDB: 12.11.2012
AB  - Previous studies with Corynebacterium diphtheriae and Mycobacterium species revealed that the transcriptional regulator DtxR and its ortholog IdeR play a central role in the control of iron metabolism. In the present work, we used genome-based approaches to determine the DtxR regulon of Corynebacterium glutamicum, a nonpathogenic relative of C. diphtheriae. First, global gene expression of a dtxR deletion mutant was compared with that of the wild type using DNA microarrays. Second, we used a computer-based approach to identify 117 putative DtxR binding sites in the C. glutamicum genome. In the third step, 74 of the corresponding genome regions were amplified by PCR, 51 of which were shifted by the DtxR protein. Finally, we analyzed which of the genes preceded by a functional DtxR binding site showed altered mRNA levels in the transcriptome comparison. Fifty-one genes organized in 27 putative operons displayed an increased mRNA level in the DeltadtxR mutant and thus are presumably repressed by DtxR. The majority of these genes are obviously involved in iron acquisition, three encode transcriptional regulators, e.g., the recently identified repressor of iron proteins RipA, and the others encode proteins of diverse or unknown functions. Thirteen genes showed a decreased mRNA level in the DeltadtxR mutant and thus might be activated by DtxR. This group included the suf operon, whose products are involved in the formation and repair of iron-sulfur clusters, and several genes for transcriptional regulators. Our results clearly establish DtxR as the master regulator of iron-dependent gene expression in C. glutamicum.
KW  - Bacterial Proteins: genetics
KW  - Bacterial Proteins: physiology
KW  - Binding Sites
KW  - Computational Biology
KW  - Corynebacterium glutamicum: genetics
KW  - Corynebacterium glutamicum: physiology
KW  - DNA, Bacterial: metabolism
KW  - DNA-Binding Proteins: genetics
KW  - DNA-Binding Proteins: physiology
KW  - Electrophoretic Mobility Shift Assay
KW  - Gene Deletion
KW  - Gene Expression Regulation, Bacterial
KW  - Iron: metabolism
KW  - Oligonucleotide Array Sequence Analysis
KW  - Operon
KW  - Protein Binding
KW  - RNA, Bacterial: analysis
KW  - RNA, Messenger: analysis
KW  - Regulon
KW  - Bacterial Proteins (NLM Chemicals)
KW  - DNA, Bacterial (NLM Chemicals)
KW  - DNA-Binding Proteins (NLM Chemicals)
KW  - RNA, Bacterial (NLM Chemicals)
KW  - RNA, Messenger (NLM Chemicals)
KW  - Iron (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:16585752
C2  - pmc:PMC1446976
UR  - <Go to ISI:>//WOS:000236746200018
DO  - DOI:10.1128/JB.188.8.2907-2918.2006
UR  - https://juser.fz-juelich.de/record/50945
ER  -