% IMPORTANT: The following is UTF-8 encoded.  This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.

@ARTICLE{Wennerhold:50945,
      author       = {Wennerhold, J. and Bott, M.},
      title        = {{T}he {D}tx{R} {R}egulon of {C}orynebacterium glutamicum},
      journal      = {Journal of bacteriology},
      volume       = {188},
      issn         = {0021-9193},
      address      = {Washington, DC},
      publisher    = {Soc.},
      reportid     = {PreJuSER-50945},
      pages        = {2907 - 2918},
      year         = {2006},
      note         = {Record converted from VDB: 12.11.2012},
      abstract     = {Previous studies with Corynebacterium diphtheriae and
                      Mycobacterium species revealed that the transcriptional
                      regulator DtxR and its ortholog IdeR play a central role in
                      the control of iron metabolism. In the present work, we used
                      genome-based approaches to determine the DtxR regulon of
                      Corynebacterium glutamicum, a nonpathogenic relative of C.
                      diphtheriae. First, global gene expression of a dtxR
                      deletion mutant was compared with that of the wild type
                      using DNA microarrays. Second, we used a computer-based
                      approach to identify 117 putative DtxR binding sites in the
                      C. glutamicum genome. In the third step, 74 of the
                      corresponding genome regions were amplified by PCR, 51 of
                      which were shifted by the DtxR protein. Finally, we analyzed
                      which of the genes preceded by a functional DtxR binding
                      site showed altered mRNA levels in the transcriptome
                      comparison. Fifty-one genes organized in 27 putative operons
                      displayed an increased mRNA level in the DeltadtxR mutant
                      and thus are presumably repressed by DtxR. The majority of
                      these genes are obviously involved in iron acquisition,
                      three encode transcriptional regulators, e.g., the recently
                      identified repressor of iron proteins RipA, and the others
                      encode proteins of diverse or unknown functions. Thirteen
                      genes showed a decreased mRNA level in the DeltadtxR mutant
                      and thus might be activated by DtxR. This group included the
                      suf operon, whose products are involved in the formation and
                      repair of iron-sulfur clusters, and several genes for
                      transcriptional regulators. Our results clearly establish
                      DtxR as the master regulator of iron-dependent gene
                      expression in C. glutamicum.},
      keywords     = {Bacterial Proteins: genetics / Bacterial Proteins:
                      physiology / Binding Sites / Computational Biology /
                      Corynebacterium glutamicum: genetics / Corynebacterium
                      glutamicum: physiology / DNA, Bacterial: metabolism /
                      DNA-Binding Proteins: genetics / DNA-Binding Proteins:
                      physiology / Electrophoretic Mobility Shift Assay / Gene
                      Deletion / Gene Expression Regulation, Bacterial / Iron:
                      metabolism / Oligonucleotide Array Sequence Analysis /
                      Operon / Protein Binding / RNA, Bacterial: analysis / RNA,
                      Messenger: analysis / Regulon / Bacterial Proteins (NLM
                      Chemicals) / DNA, Bacterial (NLM Chemicals) / DNA-Binding
                      Proteins (NLM Chemicals) / RNA, Bacterial (NLM Chemicals) /
                      RNA, Messenger (NLM Chemicals) / Iron (NLM Chemicals) / J
                      (WoSType)},
      cin          = {IBT-1},
      ddc          = {570},
      cid          = {I:(DE-Juel1)VDB55},
      pnm          = {Biotechnologie},
      pid          = {G:(DE-Juel1)FUEK410},
      shelfmark    = {Microbiology},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:16585752},
      pmc          = {pmc:PMC1446976},
      UT           = {WOS:000236746200018},
      doi          = {10.1128/JB.188.8.2907-2918.2006},
      url          = {https://juser.fz-juelich.de/record/50945},
}