Home > Publications database > The DtxR Regulon of Corynebacterium glutamicum > print

001     50945
005     20200423204323.0
024 7 _ |a pmid:16585752
|2 pmid
024 7 _ |a pmc:PMC1446976
|2 pmc
024 7 _ |a 10.1128/JB.188.8.2907-2918.2006
|2 DOI
024 7 _ |a WOS:000236746200018
|2 WOS
024 7 _ |a 2128/2428
|2 Handle
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|2 altmetric
037 _ _ |a PreJuSER-50945
041 _ _ |a eng
082 _ _ |a 570
084 _ _ |2 WoS
|a Microbiology
100 1 _ |a Wennerhold, J.
|b 0
|u FZJ
|0 P:(DE-Juel1)VDB57796
245 _ _ |a The DtxR Regulon of Corynebacterium glutamicum
260 _ _ |a Washington, DC
|b Soc.
|c 2006
300 _ _ |a 2907 - 2918
336 7 _ |a Journal Article
|0 PUB:(DE-HGF)16
|2 PUB:(DE-HGF)
336 7 _ |a Output Types/Journal article
|2 DataCite
336 7 _ |a Journal Article
|0 0
|2 EndNote
336 7 _ |a ARTICLE
|2 BibTeX
336 7 _ |a JOURNAL_ARTICLE
|2 ORCID
336 7 _ |a article
|2 DRIVER
440 _ 0 |a Journal of Bacteriology
|x 0021-9193
|0 3082
|y 8
|v 188
500 _ _ |a Record converted from VDB: 12.11.2012
520 _ _ |a Previous studies with Corynebacterium diphtheriae and Mycobacterium species revealed that the transcriptional regulator DtxR and its ortholog IdeR play a central role in the control of iron metabolism. In the present work, we used genome-based approaches to determine the DtxR regulon of Corynebacterium glutamicum, a nonpathogenic relative of C. diphtheriae. First, global gene expression of a dtxR deletion mutant was compared with that of the wild type using DNA microarrays. Second, we used a computer-based approach to identify 117 putative DtxR binding sites in the C. glutamicum genome. In the third step, 74 of the corresponding genome regions were amplified by PCR, 51 of which were shifted by the DtxR protein. Finally, we analyzed which of the genes preceded by a functional DtxR binding site showed altered mRNA levels in the transcriptome comparison. Fifty-one genes organized in 27 putative operons displayed an increased mRNA level in the DeltadtxR mutant and thus are presumably repressed by DtxR. The majority of these genes are obviously involved in iron acquisition, three encode transcriptional regulators, e.g., the recently identified repressor of iron proteins RipA, and the others encode proteins of diverse or unknown functions. Thirteen genes showed a decreased mRNA level in the DeltadtxR mutant and thus might be activated by DtxR. This group included the suf operon, whose products are involved in the formation and repair of iron-sulfur clusters, and several genes for transcriptional regulators. Our results clearly establish DtxR as the master regulator of iron-dependent gene expression in C. glutamicum.
536 _ _ |a Biotechnologie
|c PBT
|2 G:(DE-HGF)
|0 G:(DE-Juel1)FUEK410
|x 0
588 _ _ |a Dataset connected to Web of Science, Pubmed
650 _ 2 |2 MeSH
|a Bacterial Proteins: genetics
650 _ 2 |2 MeSH
|a Bacterial Proteins: physiology
650 _ 2 |2 MeSH
|a Binding Sites
650 _ 2 |2 MeSH
|a Computational Biology
650 _ 2 |2 MeSH
|a Corynebacterium glutamicum: genetics
650 _ 2 |2 MeSH
|a Corynebacterium glutamicum: physiology
650 _ 2 |2 MeSH
|a DNA, Bacterial: metabolism
650 _ 2 |2 MeSH
|a DNA-Binding Proteins: genetics
650 _ 2 |2 MeSH
|a DNA-Binding Proteins: physiology
650 _ 2 |2 MeSH
|a Electrophoretic Mobility Shift Assay
650 _ 2 |2 MeSH
|a Gene Deletion
650 _ 2 |2 MeSH
|a Gene Expression Regulation, Bacterial
650 _ 2 |2 MeSH
|a Iron: metabolism
650 _ 2 |2 MeSH
|a Oligonucleotide Array Sequence Analysis
650 _ 2 |2 MeSH
|a Operon
650 _ 2 |2 MeSH
|a Protein Binding
650 _ 2 |2 MeSH
|a RNA, Bacterial: analysis
650 _ 2 |2 MeSH
|a RNA, Messenger: analysis
650 _ 2 |2 MeSH
|a Regulon
650 _ 7 |0 0
|2 NLM Chemicals
|a Bacterial Proteins
650 _ 7 |0 0
|2 NLM Chemicals
|a DNA, Bacterial
650 _ 7 |0 0
|2 NLM Chemicals
|a DNA-Binding Proteins
650 _ 7 |0 0
|2 NLM Chemicals
|a RNA, Bacterial
650 _ 7 |0 0
|2 NLM Chemicals
|a RNA, Messenger
650 _ 7 |0 7439-89-6
|2 NLM Chemicals
|a Iron
650 _ 7 |a J
|2 WoSType
700 1 _ |a Bott, M.
|b 1
|u FZJ
|0 P:(DE-Juel1)128943
773 _ _ |a 10.1128/JB.188.8.2907-2918.2006
|g Vol. 188, p. 2907 - 2918
|p 2907 - 2918
|q 188<2907 - 2918
|0 PERI:(DE-600)1481988-0
|t Journal of bacteriology
|v 188
|y 2006
|x 0021-9193
856 7 _ |2 Pubmed Central
|u http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1446976
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