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@ARTICLE{Schnke:5102,
author = {Schünke, S. and Stoldt, M. and Novak, K. and Kaupp, U. B.
and Willbold, D.},
title = {{S}olution structure of the {M}esorhizobium loti {K}1
channel cyclic nucleotide-binding domain in complex with
c{AMP}},
journal = {EMBO reports},
volume = {10},
issn = {1469-221X},
address = {London [u.a.]},
publisher = {Nature Publishing Group},
reportid = {PreJuSER-5102},
pages = {729 - 735},
year = {2009},
note = {This study was supported by a research grant from the
Helmholtzgemeinschaft},
abstract = {Cyclic nucleotide-sensitive ion channels, known as HCN and
CNG channels, are crucial in neuronal excitability and
signal transduction of sensory cells. HCN and CNG channels
are activated by binding of cyclic nucleotides to their
intracellular cyclic nucleotide-binding domain (CNBD).
However, the mechanism by which the binding of cyclic
nucleotides opens these channels is not well understood.
Here, we report the solution structure of the isolated CNBD
of a cyclic nucleotide-sensitive K(+) channel from
Mesorhizobium loti. The protein consists of a wide
anti-parallel beta-roll topped by a helical bundle
comprising five alpha-helices and a short 3(10)-helix. In
contrast to the dimeric arrangement ('dimer-of-dimers') in
the crystal structure, the solution structure clearly shows
a monomeric fold. The monomeric structure of the CNBD
supports the hypothesis that the CNBDs transmit the binding
signal to the channel pore independently of each other.},
keywords = {Alphaproteobacteria: chemistry / Crystallography, X-Ray /
Cyclic AMP: chemistry / Cyclic AMP: metabolism / Cyclic
Nucleotide-Gated Cation Channels: chemistry / Cyclic
Nucleotide-Gated Cation Channels: metabolism / Models,
Molecular / Potassium Channels: chemistry / Potassium
Channels: metabolism / Protein Structure, Secondary /
Protein Structure, Tertiary / Solutions / Cyclic
Nucleotide-Gated Cation Channels (NLM Chemicals) / Potassium
Channels (NLM Chemicals) / Solutions (NLM Chemicals) /
Cyclic AMP (NLM Chemicals) / J (WoSType)},
cin = {ISB-1 / ISB-3 / JARA-HPC},
ddc = {570},
cid = {I:(DE-Juel1)VDB922 / I:(DE-Juel1)VDB942 /
$I:(DE-82)080012_20140620$},
pnm = {Programm Biosoft / Funktion und Dysfunktion des
Nervensystems},
pid = {G:(DE-Juel1)FUEK443 / G:(DE-Juel1)FUEK409},
shelfmark = {Biochemistry $\&$ Molecular Biology / Cell Biology},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:19465888},
pmc = {pmc:PMC2727438},
UT = {WOS:000267599700015},
doi = {10.1038/embor.2009.68},
url = {https://juser.fz-juelich.de/record/5102},
}