Hauptseite > Publikationsdatenbank > Postnatal expression pattern of HCN channel isoforms in thalamic neurons: Relationship to maturation of thalamocortical oscillations > print

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024 7 _ |2 pmid
|a pmid:19587292
024 7 _ |2 pmc
|a pmc:PMC2768285
024 7 _ |2 DOI
|a 10.1523/JNEUROSCI.0689-09.2009
024 7 _ |2 WOS
|a WOS:000267818400026
037 _ _ |a PreJuSER-5103
041 _ _ |a eng
082 _ _ |a 590
084 _ _ |2 WoS
|a Neurosciences
100 1 _ |0 P:(DE-HGF)0
|a Kanyshkova, T.
|b 0
245 _ _ |a Postnatal expression pattern of HCN channel isoforms in thalamic neurons: Relationship to maturation of thalamocortical oscillations
260 _ _ |a Washington, DC
|b Soc.
|c 2009
300 _ _ |a 8857 - 8847
336 7 _ |a Journal Article
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336 7 _ |a Output Types/Journal article
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336 7 _ |a Journal Article
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336 7 _ |a ARTICLE
|2 BibTeX
336 7 _ |a JOURNAL_ARTICLE
|2 ORCID
336 7 _ |a article
|2 DRIVER
440 _ 0 |0 3603
|a Journal of Neuroscience
|v 29
|x 0270-6474
|y 27
500 _ _ |a This work was supported by Deutsche Forschungsgemeinschaft Grants BU 1019/8-1 and BE 4107/2-1, Interdisziplinares Zentrum fur Klinische Forschung Grant Bud/005/07, and National Institutes of Health Grants NS 35439 and NS 45540. This work was done in partial fulfillment of the MD thesis of M. P. and the PhD thesis of P. M. We thank A. Markovic, E. Nass, and S. Ruppel for excellent technical assistance.
520 _ _ |a Hyperpolarization-activated cyclic nucleotide-gated cation (HCN) channels are the molecular substrate of the hyperpolarization-activated inward current (I(h)). Because the developmental profile of HCN channels in the thalamus is not well understood, we combined electrophysiological, molecular, immunohistochemical, EEG recordings in vivo, and computer modeling techniques to examine HCN gene expression and I(h) properties in rat thalamocortical relay (TC) neurons in the dorsal part of the lateral geniculate nucleus and the functional consequence of this maturation. Recordings of TC neurons revealed an approximate sixfold increase in I(h) density between postnatal day 3 (P3) and P106, which was accompanied by significantly altered current kinetics, cAMP sensitivity, and steady-state activation properties. Quantification on tissue levels revealed a significant developmental decrease in cAMP. Consequently the block of basal adenylyl cyclase activity was accompanied by a hyperpolarizing shift of the I(h) activation curve in young but not adult rats. Quantitative analyses of HCN channel isoforms revealed a steady increase of mRNA and protein expression levels of HCN1, HCN2, and HCN4 with reduced relative abundance of HCN4. Computer modeling in a simplified thalamic network indicated that the occurrence of rhythmic delta activity, which was present in the EEG at P12, differentially depended on I(h) conductance and modulation by cAMP at different developmental states. These data indicate that the developmental increase in I(h) density results from increased expression of three HCN channel isoforms and that isoform composition and intracellular cAMP levels interact in determining I(h) properties to enable progressive maturation of rhythmic slow-wave sleep activity patterns.
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588 _ _ |a Dataset connected to Web of Science, Pubmed
650 _ 2 |2 MeSH
|a Animals
650 _ 2 |2 MeSH
|a Animals, Newborn
650 _ 2 |2 MeSH
|a Biological Clocks: physiology
650 _ 2 |2 MeSH
|a Cerebral Cortex: growth & development
650 _ 2 |2 MeSH
|a Cerebral Cortex: metabolism
650 _ 2 |2 MeSH
|a Cyclic Nucleotide-Gated Cation Channels: biosynthesis
650 _ 2 |2 MeSH
|a Cyclic Nucleotide-Gated Cation Channels: genetics
650 _ 2 |2 MeSH
|a Gene Expression Regulation, Developmental: physiology
650 _ 2 |2 MeSH
|a Ion Channels: biosynthesis
650 _ 2 |2 MeSH
|a Ion Channels: genetics
650 _ 2 |2 MeSH
|a Neural Pathways: growth & development
650 _ 2 |2 MeSH
|a Neural Pathways: metabolism
650 _ 2 |2 MeSH
|a Neurons: metabolism
650 _ 2 |2 MeSH
|a Neurons: physiology
650 _ 2 |2 MeSH
|a Potassium Channels: biosynthesis
650 _ 2 |2 MeSH
|a Potassium Channels: genetics
650 _ 2 |2 MeSH
|a Protein Isoforms: biosynthesis
650 _ 2 |2 MeSH
|a Rats
650 _ 2 |2 MeSH
|a Rats, Sprague-Dawley
650 _ 2 |2 MeSH
|a Thalamus: growth & development
650 _ 2 |2 MeSH
|a Thalamus: metabolism
650 _ 7 |0 0
|2 NLM Chemicals
|a Cyclic Nucleotide-Gated Cation Channels
650 _ 7 |0 0
|2 NLM Chemicals
|a HCN2 potassium channel
650 _ 7 |0 0
|2 NLM Chemicals
|a HCN3 protein, rat
650 _ 7 |0 0
|2 NLM Chemicals
|a HCN4 protein, rat
650 _ 7 |0 0
|2 NLM Chemicals
|a Ion Channels
650 _ 7 |0 0
|2 NLM Chemicals
|a Potassium Channels
650 _ 7 |0 0
|2 NLM Chemicals
|a Protein Isoforms
650 _ 7 |0 0
|2 NLM Chemicals
|a hyperpolarization-activated cation channel
650 _ 7 |2 WoSType
|a J
700 1 _ |0 P:(DE-HGF)0
|a Pawlowski, M.
|b 1
700 1 _ |0 P:(DE-HGF)0
|a Meuth, P.
|b 2
700 1 _ |0 P:(DE-HGF)0
|a Dube, C.
|b 3
700 1 _ |0 P:(DE-HGF)0
|a Bender, R. A.
|b 4
700 1 _ |0 P:(DE-HGF)0
|a Brewster, A. L..
|b 5
700 1 _ |0 P:(DE-Juel1)131911
|a Baumann, A.
|b 6
|u FZJ
700 1 _ |0 P:(DE-HGF)0
|a Baram, T. Z.
|b 7
700 1 _ |0 P:(DE-HGF)0
|a Pape, H.-C.
|b 8
700 1 _ |0 P:(DE-HGF)0
|a Budde, T.
|b 9
773 _ _ |0 PERI:(DE-600)1475274-8
|a 10.1523/JNEUROSCI.0689-09.2009
|g Vol. 29, p. 8857 - 8847
|p 8857 - 8847
|q 29<8857 - 8847
|t The @journal of neuroscience
|v 29
|x 0270-6474
|y 2009
856 7 _ |2 Pubmed Central
|u http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2768285
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