% IMPORTANT: The following is UTF-8 encoded.  This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.

@ARTICLE{Kriegsmann:5165,
      author       = {Kriegsmann, J. and Gregor, I. and von der Hocht, I. and
                      Klare, J. and Engelhard, M. and Enderlein, J. and Fitter,
                      J.},
      title        = {{T}ranslational {D}iffusion and {I}nteraction of a
                      {P}hotoreceptor and {I}ts {C}ognate {T}ransducer {O}bserved
                      in {G}iant {U}nilamellar {V}esicles by {U}sing
                      {D}ual-{F}ocus {FCS}},
      journal      = {ChemBioChem},
      volume       = {10},
      issn         = {1439-4227},
      address      = {Weinheim},
      publisher    = {Wiley-VCH},
      reportid     = {PreJuSER-5165},
      pages        = {1823 - 1829},
      year         = {2009},
      note         = {The priority program SPP 7128 of Deutsche
                      Forschungsgemeinschaft (F1 84113-1,2 to J.F and to J.E) and
                      the DFG graduate school 1035 "Biointerface" are acknowledged
                      for funding. We thank B. Kaupp and G. Baldt for continuous
                      support in their institutes.},
      abstract     = {In order to monitor membrane-protein binding in lipid
                      bilayers at physiological protein concentrations, we
                      employed the recently developed dual-focus fluorescence
                      correlation spectroscopy (2fFCS) technique. In a case study
                      on a photoreceptor consisting of seven transmembrane helices
                      and its cognate transducer (two transmembrane helices), the
                      lateral diffusion for these integral membrane proteins was
                      analyzed in giant unilamellar vesicles (GUVs). The
                      two-dimensional diffusion coefficients of both separately
                      diffusing proteins differ significantly, with D = 2.2 x
                      10(-8) cm2 s(-1) for the photoreceptor and with D = 4.1 x
                      10(-8) cm2 s(-1) for the transducer. In GUVs with both
                      membrane proteins present together, we observed
                      significantly smaller diffusion coefficients for labelled
                      transducer molecules; this indicates the presence of larger
                      diffusing units and therefore intermolecular protein
                      binding. Based on the phenomenological dependence of
                      diffusion coefficients on the molecule's cylindrical radius,
                      we are able to estimate the degree of membrane protein
                      binding on a quantitative level.},
      keywords     = {Archaeal Proteins: chemistry / Archaeal Proteins:
                      metabolism / Carotenoids: chemistry / Carotenoids:
                      metabolism / Diffusion / Lipid Bilayers: chemistry /
                      Membrane Proteins: chemistry / Photoreceptors, Microbial:
                      chemistry / Photoreceptors, Microbial: metabolism / Protein
                      Binding / Spectrometry, Fluorescence / Unilamellar
                      Liposomes: chemistry / Archaeal Proteins (NLM Chemicals) /
                      HtrII protein, Natronobacterium pharaonis (NLM Chemicals) /
                      Lipid Bilayers (NLM Chemicals) / Membrane Proteins (NLM
                      Chemicals) / Photoreceptors, Microbial (NLM Chemicals) /
                      Unilamellar Liposomes (NLM Chemicals) / phototaxis receptor
                      sensory rhodopsin II, Natronobacterium pharaonis (NLM
                      Chemicals) / Carotenoids (NLM Chemicals) / J (WoSType)},
      cin          = {ISB-2},
      ddc          = {540},
      cid          = {I:(DE-Juel1)ISB-2-20090406},
      pnm          = {Programm Biosoft},
      pid          = {G:(DE-Juel1)FUEK443},
      shelfmark    = {Biochemistry $\&$ Molecular Biology / Chemistry, Medicinal},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:19551796},
      UT           = {WOS:000268504500015},
      doi          = {10.1002/cbic.200900251},
      url          = {https://juser.fz-juelich.de/record/5165},
}