guest ::
| Home > Publications database > Translational Diffusion and Interaction of a Photoreceptor and Its Cognate Transducer Observed in Giant Unilamellar Vesicles by Using Dual-Focus FCS > print |
| Home > Publications database > Translational Diffusion and Interaction of a Photoreceptor and Its Cognate Transducer Observed in Giant Unilamellar Vesicles by Using Dual-Focus FCS > print |
| 001 | 5165 | ||
| 005 | 20200402205627.0 | ||
| 024 | 7 | _ | |2 pmid |a pmid:19551796 |
| 024 | 7 | _ | |2 DOI |a 10.1002/cbic.200900251 |
| 024 | 7 | _ | |2 WOS |a WOS:000268504500015 |
| 037 | _ | _ | |a PreJuSER-5165 |
| 041 | _ | _ | |a eng |
| 082 | _ | _ | |a 540 |
| 084 | _ | _ | |2 WoS |a Biochemistry & Molecular Biology |
| 084 | _ | _ | |2 WoS |a Chemistry, Medicinal |
| 100 | 1 | _ | |0 P:(DE-Juel1)VDB60877 |a Kriegsmann, J. |b 0 |u FZJ |
| 245 | _ | _ | |a Translational Diffusion and Interaction of a Photoreceptor and Its Cognate Transducer Observed in Giant Unilamellar Vesicles by Using Dual-Focus FCS |
| 260 | _ | _ | |a Weinheim |b Wiley-VCH |c 2009 |
| 300 | _ | _ | |a 1823 - 1829 |
| 336 | 7 | _ | |a Journal Article |0 PUB:(DE-HGF)16 |2 PUB:(DE-HGF) |
| 336 | 7 | _ | |a Output Types/Journal article |2 DataCite |
| 336 | 7 | _ | |a Journal Article |0 0 |2 EndNote |
| 336 | 7 | _ | |a ARTICLE |2 BibTeX |
| 336 | 7 | _ | |a JOURNAL_ARTICLE |2 ORCID |
| 336 | 7 | _ | |a article |2 DRIVER |
| 440 | _ | 0 | |0 8962 |a ChemBioChem |v 10 |x 1439-4227 |y 11 |
| 500 | _ | _ | |a The priority program SPP 7128 of Deutsche Forschungsgemeinschaft (F1 84113-1,2 to J.F and to J.E) and the DFG graduate school 1035 "Biointerface" are acknowledged for funding. We thank B. Kaupp and G. Baldt for continuous support in their institutes. |
| 520 | _ | _ | |a In order to monitor membrane-protein binding in lipid bilayers at physiological protein concentrations, we employed the recently developed dual-focus fluorescence correlation spectroscopy (2fFCS) technique. In a case study on a photoreceptor consisting of seven transmembrane helices and its cognate transducer (two transmembrane helices), the lateral diffusion for these integral membrane proteins was analyzed in giant unilamellar vesicles (GUVs). The two-dimensional diffusion coefficients of both separately diffusing proteins differ significantly, with D = 2.2 x 10(-8) cm2 s(-1) for the photoreceptor and with D = 4.1 x 10(-8) cm2 s(-1) for the transducer. In GUVs with both membrane proteins present together, we observed significantly smaller diffusion coefficients for labelled transducer molecules; this indicates the presence of larger diffusing units and therefore intermolecular protein binding. Based on the phenomenological dependence of diffusion coefficients on the molecule's cylindrical radius, we are able to estimate the degree of membrane protein binding on a quantitative level. |
| 536 | _ | _ | |0 G:(DE-Juel1)FUEK443 |2 G:(DE-HGF) |a Programm Biosoft |c N03 |x 0 |
| 588 | _ | _ | |a Dataset connected to Web of Science, Pubmed |
| 650 | _ | 2 | |2 MeSH |a Archaeal Proteins: chemistry |
| 650 | _ | 2 | |2 MeSH |a Archaeal Proteins: metabolism |
| 650 | _ | 2 | |2 MeSH |a Carotenoids: chemistry |
| 650 | _ | 2 | |2 MeSH |a Carotenoids: metabolism |
| 650 | _ | 2 | |2 MeSH |a Diffusion |
| 650 | _ | 2 | |2 MeSH |a Lipid Bilayers: chemistry |
| 650 | _ | 2 | |2 MeSH |a Membrane Proteins: chemistry |
| 650 | _ | 2 | |2 MeSH |a Photoreceptors, Microbial: chemistry |
| 650 | _ | 2 | |2 MeSH |a Photoreceptors, Microbial: metabolism |
| 650 | _ | 2 | |2 MeSH |a Protein Binding |
| 650 | _ | 2 | |2 MeSH |a Spectrometry, Fluorescence |
| 650 | _ | 2 | |2 MeSH |a Unilamellar Liposomes: chemistry |
| 650 | _ | 7 | |0 0 |2 NLM Chemicals |a Archaeal Proteins |
| 650 | _ | 7 | |0 0 |2 NLM Chemicals |a HtrII protein, Natronobacterium pharaonis |
| 650 | _ | 7 | |0 0 |2 NLM Chemicals |a Lipid Bilayers |
| 650 | _ | 7 | |0 0 |2 NLM Chemicals |a Membrane Proteins |
| 650 | _ | 7 | |0 0 |2 NLM Chemicals |a Photoreceptors, Microbial |
| 650 | _ | 7 | |0 0 |2 NLM Chemicals |a Unilamellar Liposomes |
| 650 | _ | 7 | |0 0 |2 NLM Chemicals |a phototaxis receptor sensory rhodopsin II, Natronobacterium pharaonis |
| 650 | _ | 7 | |0 36-88-4 |2 NLM Chemicals |a Carotenoids |
| 650 | _ | 7 | |2 WoSType |a J |
| 653 | 2 | 0 | |2 Author |a fluorescence spectroscopy |
| 653 | 2 | 0 | |2 Author |a giant unilamellar vesicles |
| 653 | 2 | 0 | |2 Author |a membrane proteins |
| 653 | 2 | 0 | |2 Author |a photo-signaling complexes |
| 653 | 2 | 0 | |2 Author |a protein diffusion |
| 700 | 1 | _ | |0 P:(DE-HGF)0 |a Gregor, I. |b 1 |
| 700 | 1 | _ | |0 P:(DE-HGF)0 |a von der Hocht, I. |b 2 |
| 700 | 1 | _ | |0 P:(DE-HGF)0 |a Klare, J. |b 3 |
| 700 | 1 | _ | |0 P:(DE-HGF)0 |a Engelhard, M. |b 4 |
| 700 | 1 | _ | |0 P:(DE-HGF)0 |a Enderlein, J. |b 5 |
| 700 | 1 | _ | |0 P:(DE-Juel1)131961 |a Fitter, J. |b 6 |u FZJ |
| 773 | _ | _ | |0 PERI:(DE-600)2020469-3 |a 10.1002/cbic.200900251 |g Vol. 10, p. 1823 - 1829 |p 1823 - 1829 |q 10<1823 - 1829 |t ChemBioChem |v 10 |x 1439-4227 |y 2009 |
| 856 | 7 | _ | |u http://dx.doi.org/10.1002/cbic.200900251 |
| 909 | C | O | |o oai:juser.fz-juelich.de:5165 |p VDB |
| 913 | 1 | _ | |0 G:(DE-Juel1)FUEK443 |a DE-HGF |b Schlüsseltechnologien |k N03 |l BioSoft |v Programm Biosoft |x 0 |z entfällt |
| 914 | 1 | _ | |y 2009 |
| 915 | _ | _ | |0 StatID:(DE-HGF)0010 |a JCR/ISI refereed |
| 920 | 1 | _ | |0 I:(DE-Juel1)ISB-2-20090406 |d 31.12.2010 |g ISB |k ISB-2 |l Molekulare Biophysik |x 0 |
| 970 | _ | _ | |a VDB:(DE-Juel1)112623 |
| 980 | _ | _ | |a VDB |
| 980 | _ | _ | |a ConvertedRecord |
| 980 | _ | _ | |a journal |
| 980 | _ | _ | |a I:(DE-Juel1)ICS-6-20110106 |
| 980 | _ | _ | |a UNRESTRICTED |
| 981 | _ | _ | |a I:(DE-Juel1)IBI-7-20200312 |
| 981 | _ | _ | |a I:(DE-Juel1)ICS-6-20110106 |
| 981 | _ | _ | |a I:(DE-Juel1)ISB-2-20090406 |
| Library | Collection | CLSMajor | CLSMinor | Language | Author |
|---|