TY  - JOUR
AU  - Thielmann, Y.
AU  - Weiergräber, O.H.
AU  - Mohrlüder, J.
AU  - Willbold, D.
TI  - Structural framework of the GABARAP-calreticulin interface - implications for substrate binding to endoplasmic reticulum chaperones
JO  - The FEBS journal
VL  - 276
SN  - 1742-464X
CY  - Oxford [u.a.]
PB  - Wiley-Blackwell
M1  - PreJuSER-5170
SP  - 1140 - 1152
PY  - 2009
N1  - The authors wish to thank Olga Dietz for excellent technical assistance. O. H. WeiergrAber is grateful to Georg Buldt for continuous generous support. Moreover, assistance by the ESRF staff at beamline ID14-1 is acknowledged. This study was supported by a research grant from the Deutsche Forschungsgemeinschaft (DFG) to D. Willbold (Wi1472/5).
AB  - The 4-aminobutyrate type A receptor-associated protein (GABARAP) is a versatile adaptor protein that plays an important role in intracellular vesicle trafficking, particularly in neuronal cells. We have investigated the structural determinants underlying the interaction of GABARAP with calreticulin using spectroscopic and crystallographic techniques. Specifically, we present the crystal structure of GABARAP in complex with its major binding epitope on the chaperone. Molecular modeling of a complex containing full-length calreticulin suggests a novel mode of substrate interaction, which may have functional implications for the calreticulin/calnexin family in general.
KW  - Adaptor Proteins, Signal Transducing: chemistry
KW  - Adaptor Proteins, Signal Transducing: metabolism
KW  - Calreticulin: chemistry
KW  - Calreticulin: metabolism
KW  - Endoplasmic Reticulum: metabolism
KW  - Humans
KW  - Microtubule-Associated Proteins: chemistry
KW  - Microtubule-Associated Proteins: metabolism
KW  - Models, Molecular
KW  - Molecular Chaperones: chemistry
KW  - Molecular Chaperones: metabolism
KW  - Protein Binding
KW  - Substrate Specificity
KW  - Adaptor Proteins, Signal Transducing (NLM Chemicals)
KW  - Calreticulin (NLM Chemicals)
KW  - GABARAP protein, human (NLM Chemicals)
KW  - Microtubule-Associated Proteins (NLM Chemicals)
KW  - Molecular Chaperones (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:19154346
UR  - <Go to ISI:>//WOS:000262666900022
DO  - DOI:10.1111/j.1742-4658.2008.06857.x
UR  - https://juser.fz-juelich.de/record/5170
ER  -