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@ARTICLE{Thielmann:5170,
author = {Thielmann, Y. and Weiergräber, O.H. and Mohrlüder, J. and
Willbold, D.},
title = {{S}tructural framework of the {GABARAP}-calreticulin
interface - implications for substrate binding to
endoplasmic reticulum chaperones},
journal = {The FEBS journal},
volume = {276},
issn = {1742-464X},
address = {Oxford [u.a.]},
publisher = {Wiley-Blackwell},
reportid = {PreJuSER-5170},
pages = {1140 - 1152},
year = {2009},
note = {The authors wish to thank Olga Dietz for excellent
technical assistance. O. H. WeiergrAber is grateful to Georg
Buldt for continuous generous support. Moreover, assistance
by the ESRF staff at beamline ID14-1 is acknowledged. This
study was supported by a research grant from the Deutsche
Forschungsgemeinschaft (DFG) to D. Willbold (Wi1472/5).},
abstract = {The 4-aminobutyrate type A receptor-associated protein
(GABARAP) is a versatile adaptor protein that plays an
important role in intracellular vesicle trafficking,
particularly in neuronal cells. We have investigated the
structural determinants underlying the interaction of
GABARAP with calreticulin using spectroscopic and
crystallographic techniques. Specifically, we present the
crystal structure of GABARAP in complex with its major
binding epitope on the chaperone. Molecular modeling of a
complex containing full-length calreticulin suggests a novel
mode of substrate interaction, which may have functional
implications for the calreticulin/calnexin family in
general.},
keywords = {Adaptor Proteins, Signal Transducing: chemistry / Adaptor
Proteins, Signal Transducing: metabolism / Calreticulin:
chemistry / Calreticulin: metabolism / Endoplasmic
Reticulum: metabolism / Humans / Microtubule-Associated
Proteins: chemistry / Microtubule-Associated Proteins:
metabolism / Models, Molecular / Molecular Chaperones:
chemistry / Molecular Chaperones: metabolism / Protein
Binding / Substrate Specificity / Adaptor Proteins, Signal
Transducing (NLM Chemicals) / Calreticulin (NLM Chemicals) /
GABARAP protein, human (NLM Chemicals) /
Microtubule-Associated Proteins (NLM Chemicals) / Molecular
Chaperones (NLM Chemicals) / J (WoSType)},
cin = {ISB-2 / JARA-HPC},
ddc = {540},
cid = {I:(DE-Juel1)ISB-2-20090406 / $I:(DE-82)080012_20140620$},
pnm = {Programm Biosoft},
pid = {G:(DE-Juel1)FUEK443},
shelfmark = {Biochemistry $\&$ Molecular Biology},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:19154346},
UT = {WOS:000262666900022},
doi = {10.1111/j.1742-4658.2008.06857.x},
url = {https://juser.fz-juelich.de/record/5170},
}