%0 Journal Article
%A Block, H.
%A Kubicek, J.
%A Labahn, J.
%A Roth, U.
%A Schaefer, F.
%T Production and comprehensive quality control of recombinant human interleukin-1 beta: A case study for a process development strategy
%J Protein expression and purification
%V 57
%@ 1046-5928
%C Orlando, Fla.
%I Academic Press
%M PreJuSER-5173
%P 244 - 254
%D 2008
%Z Record converted from VDB: 12.11.2012
%X We describe an efficient strategy to produce high-quality proteins by using a single large IMAC chromatography column and enzymatic His-tag removal via the TAGZyme system in pilot scale. Numerous quality assays demonstrated a high purity of the final product, the human cytokine Interleukin-1beta (IL-1beta). The protein preparation was apparently free of host cell proteins, endotoxins, protease, and aggregates. The N-terminal amino acid sequence of IL-1beta was in full agreement with the natural mature form of IL-1beta. The homogeneity of the product was further shown by X-ray structure determination which confirmed the previously solved structure of the protein. We propose the applied workflow as a strategy for industrial production of protein-based biopharmaceuticals.
%K Amino Acid Sequence
%K Biotechnology: methods
%K Chromatography, Affinity
%K Electrophoresis, Gel, Two-Dimensional
%K Endodeoxyribonucleases: isolation & purification
%K Endoribonucleases: isolation & purification
%K Endotoxins: isolation & purification
%K Exopeptidases: isolation & purification
%K Histidine: metabolism
%K Humans
%K Interleukin-1beta: biosynthesis
%K Interleukin-1beta: chemistry
%K Interleukin-1beta: isolation & purification
%K Models, Molecular
%K Molecular Sequence Data
%K Oligopeptides: metabolism
%K Recombinant Proteins: biosynthesis
%K Recombinant Proteins: chemistry
%K Recombinant Proteins: isolation & purification
%K Sequence Analysis, Protein
%K Endotoxins (NLM Chemicals)
%K His-His-His-His-His-His (NLM Chemicals)
%K Interleukin-1beta (NLM Chemicals)
%K Oligopeptides (NLM Chemicals)
%K Recombinant Proteins (NLM Chemicals)
%K Histidine (NLM Chemicals)
%K Endodeoxyribonucleases (NLM Chemicals)
%K Endoribonucleases (NLM Chemicals)
%K Serratia marcescens nuclease (NLM Chemicals)
%K Exopeptidases (NLM Chemicals)
%K J (WoSType)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:18053740
%U <Go to ISI:>//WOS:000252976500017
%R 10.1016/j.pep.2007.09.019
%U https://juser.fz-juelich.de/record/5173