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000005173 0247_ $$2DOI$$a10.1016/j.pep.2007.09.019
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000005173 084__ $$2WoS$$aBiochemical Research Methods
000005173 084__ $$2WoS$$aBiochemistry & Molecular Biology
000005173 084__ $$2WoS$$aBiotechnology & Applied Microbiology
000005173 1001_ $$0P:(DE-HGF)0$$aBlock, H.$$b0
000005173 245__ $$aProduction and comprehensive quality control of recombinant human interleukin-1 beta: A case study for a process development strategy
000005173 260__ $$aOrlando, Fla.$$bAcademic Press$$c2008
000005173 300__ $$a244 - 254
000005173 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article
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000005173 3367_ $$2DRIVER$$aarticle
000005173 440_0 $$021001$$aProtein Expression and Purification$$v57$$x1046-5928$$y2
000005173 500__ $$aRecord converted from VDB: 12.11.2012
000005173 520__ $$aWe describe an efficient strategy to produce high-quality proteins by using a single large IMAC chromatography column and enzymatic His-tag removal via the TAGZyme system in pilot scale. Numerous quality assays demonstrated a high purity of the final product, the human cytokine Interleukin-1beta (IL-1beta). The protein preparation was apparently free of host cell proteins, endotoxins, protease, and aggregates. The N-terminal amino acid sequence of IL-1beta was in full agreement with the natural mature form of IL-1beta. The homogeneity of the product was further shown by X-ray structure determination which confirmed the previously solved structure of the protein. We propose the applied workflow as a strategy for industrial production of protein-based biopharmaceuticals.
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000005173 588__ $$aDataset connected to Web of Science, Pubmed
000005173 650_2 $$2MeSH$$aAmino Acid Sequence
000005173 650_2 $$2MeSH$$aBiotechnology: methods
000005173 650_2 $$2MeSH$$aChromatography, Affinity
000005173 650_2 $$2MeSH$$aElectrophoresis, Gel, Two-Dimensional
000005173 650_2 $$2MeSH$$aEndodeoxyribonucleases: isolation & purification
000005173 650_2 $$2MeSH$$aEndoribonucleases: isolation & purification
000005173 650_2 $$2MeSH$$aEndotoxins: isolation & purification
000005173 650_2 $$2MeSH$$aExopeptidases: isolation & purification
000005173 650_2 $$2MeSH$$aHistidine: metabolism
000005173 650_2 $$2MeSH$$aHumans
000005173 650_2 $$2MeSH$$aInterleukin-1beta: biosynthesis
000005173 650_2 $$2MeSH$$aInterleukin-1beta: chemistry
000005173 650_2 $$2MeSH$$aInterleukin-1beta: isolation & purification
000005173 650_2 $$2MeSH$$aModels, Molecular
000005173 650_2 $$2MeSH$$aMolecular Sequence Data
000005173 650_2 $$2MeSH$$aOligopeptides: metabolism
000005173 650_2 $$2MeSH$$aRecombinant Proteins: biosynthesis
000005173 650_2 $$2MeSH$$aRecombinant Proteins: chemistry
000005173 650_2 $$2MeSH$$aRecombinant Proteins: isolation & purification
000005173 650_2 $$2MeSH$$aSequence Analysis, Protein
000005173 650_7 $$00$$2NLM Chemicals$$aEndotoxins
000005173 650_7 $$00$$2NLM Chemicals$$aHis-His-His-His-His-His
000005173 650_7 $$00$$2NLM Chemicals$$aInterleukin-1beta
000005173 650_7 $$00$$2NLM Chemicals$$aOligopeptides
000005173 650_7 $$00$$2NLM Chemicals$$aRecombinant Proteins
000005173 650_7 $$071-00-1$$2NLM Chemicals$$aHistidine
000005173 650_7 $$0EC 3.1.-$$2NLM Chemicals$$aEndodeoxyribonucleases
000005173 650_7 $$0EC 3.1.-$$2NLM Chemicals$$aEndoribonucleases
000005173 650_7 $$0EC 3.1.30.2$$2NLM Chemicals$$aSerratia marcescens nuclease
000005173 650_7 $$0EC 3.4.-$$2NLM Chemicals$$aExopeptidases
000005173 650_7 $$2WoSType$$aJ
000005173 65320 $$2Author$$aInterleukin-1 beta (IL-1 beta)
000005173 65320 $$2Author$$aexpression screening
000005173 65320 $$2Author$$aprotein production
000005173 65320 $$2Author$$aX-ray crystallography
000005173 65320 $$2Author$$aTAGZyme
000005173 65320 $$2Author$$aDAPase
000005173 65320 $$2Author$$aHis-Tag cleavage
000005173 65320 $$2Author$$aImmobilized-Metal Affinity Chromatography (IMAC)
000005173 65320 $$2Author$$aNi-NTA
000005173 7001_ $$0P:(DE-HGF)0$$aKubicek, J.$$b1
000005173 7001_ $$0P:(DE-Juel1)VDB886$$aLabahn, J.$$b2$$uFZJ
000005173 7001_ $$0P:(DE-HGF)0$$aRoth, U.$$b3
000005173 7001_ $$0P:(DE-HGF)0$$aSchaefer, F.$$b4
000005173 773__ $$0PERI:(DE-600)1471688-4$$a10.1016/j.pep.2007.09.019$$gVol. 57, p. 244 - 254$$p244 - 254$$q57<244 - 254$$tProtein expression and purification$$v57$$x1046-5928$$y2008
000005173 8567_ $$uhttp://dx.doi.org/10.1016/j.pep.2007.09.019
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000005173 9201_ $$0I:(DE-Juel1)ISB-2-20090406$$d31.12.2010$$gISB$$kISB-2$$lMolekulare Biophysik$$x0
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