TY  - JOUR
AU  - Block, H.
AU  - Kubicek, J.
AU  - Labahn, J.
AU  - Roth, U.
AU  - Schaefer, F.
TI  - Production and comprehensive quality control of recombinant human interleukin-1 beta: A case study for a process development strategy
JO  - Protein expression and purification
VL  - 57
SN  - 1046-5928
CY  - Orlando, Fla.
PB  - Academic Press
M1  - PreJuSER-5173
SP  - 244 - 254
PY  - 2008
N1  - Record converted from VDB: 12.11.2012
AB  - We describe an efficient strategy to produce high-quality proteins by using a single large IMAC chromatography column and enzymatic His-tag removal via the TAGZyme system in pilot scale. Numerous quality assays demonstrated a high purity of the final product, the human cytokine Interleukin-1beta (IL-1beta). The protein preparation was apparently free of host cell proteins, endotoxins, protease, and aggregates. The N-terminal amino acid sequence of IL-1beta was in full agreement with the natural mature form of IL-1beta. The homogeneity of the product was further shown by X-ray structure determination which confirmed the previously solved structure of the protein. We propose the applied workflow as a strategy for industrial production of protein-based biopharmaceuticals.
KW  - Amino Acid Sequence
KW  - Biotechnology: methods
KW  - Chromatography, Affinity
KW  - Electrophoresis, Gel, Two-Dimensional
KW  - Endodeoxyribonucleases: isolation & purification
KW  - Endoribonucleases: isolation & purification
KW  - Endotoxins: isolation & purification
KW  - Exopeptidases: isolation & purification
KW  - Histidine: metabolism
KW  - Humans
KW  - Interleukin-1beta: biosynthesis
KW  - Interleukin-1beta: chemistry
KW  - Interleukin-1beta: isolation & purification
KW  - Models, Molecular
KW  - Molecular Sequence Data
KW  - Oligopeptides: metabolism
KW  - Recombinant Proteins: biosynthesis
KW  - Recombinant Proteins: chemistry
KW  - Recombinant Proteins: isolation & purification
KW  - Sequence Analysis, Protein
KW  - Endotoxins (NLM Chemicals)
KW  - His-His-His-His-His-His (NLM Chemicals)
KW  - Interleukin-1beta (NLM Chemicals)
KW  - Oligopeptides (NLM Chemicals)
KW  - Recombinant Proteins (NLM Chemicals)
KW  - Histidine (NLM Chemicals)
KW  - Endodeoxyribonucleases (NLM Chemicals)
KW  - Endoribonucleases (NLM Chemicals)
KW  - Serratia marcescens nuclease (NLM Chemicals)
KW  - Exopeptidases (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:18053740
UR  - <Go to ISI:>//WOS:000252976500017
DO  - DOI:10.1016/j.pep.2007.09.019
UR  - https://juser.fz-juelich.de/record/5173
ER  -