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@ARTICLE{Jahnke:52636,
author = {Jahnke, S. and Pieruschka, R.},
title = {{A}ir pressure in clamp-on leaf chambers: a neglected issue
in gas exchange measurements},
journal = {The journal of experimental botany},
volume = {57},
issn = {0022-0957},
address = {Oxford},
publisher = {Univ. Press},
reportid = {PreJuSER-52636},
pages = {2553 - 2561},
year = {2006},
note = {Record converted from VDB: 12.11.2012},
abstract = {Air pressure in leaf chambers is thought to affect gas
exchange measurements through changes in partial pressure of
the air components. However, other effects may come into
play when homobaric leaves are measured in which internal
lateral gas flow may occur. When there was no pressure
difference between the leaf chamber and ambient air
(DeltaP=0), it was found in previous work that lateral CO(2)
diffusion could affect measurements performed with clamp-on
leaf chambers. On the other hand, overpressure (DeltaP>0) in
leaf chambers has been reported to minimize artefacts
possibly caused by leaks in chamber sealing. In the present
work, net CO(2) exchange rates (NCER) were measured under
different DeltaP values (0.0-3.0 kPa) on heterobaric and
homobaric leaves. In heterobaric leaves which have internal
barriers for lateral gas movement, changes in DeltaP had no
significant effect on NCER. For homobaric leaves, effects of
DeltaP>0 on measured NCER were significant, obviously due to
lateral gas flux inside the leaf mesophyll. The magnitude of
the effect was largely defined by stomatal conductance; when
stomata were widely open, the impact of DeltaP on measured
NCER was up to 7 mumol CO(2) m(-2) s(-1) kPa(-1). Since many
other factors are also involved, neither DeltaP=0 nor
DeltaP>0 was found to be the 'one-size fits all' solution to
avoid erroneous effects of lateral gas transport on
measurements with clamp-on leaf chambers.},
keywords = {Air Pressure / Artifacts / Botany: instrumentation /
Botany: methods / Botany: standards / Carbon Dioxide:
chemistry / Carbon Dioxide: metabolism / Diffusion /
Photosynthesis / Plant Leaves: metabolism / Plant
Transpiration: physiology / Carbon Dioxide (NLM Chemicals) /
J (WoSType)},
cin = {ICG-III},
ddc = {580},
cid = {I:(DE-Juel1)VDB49},
pnm = {Terrestrische Umwelt},
pid = {G:(DE-Juel1)FUEK407},
shelfmark = {Plant Sciences},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:16820393},
UT = {WOS:000239901500009},
doi = {10.1093/jxb/erl003},
url = {https://juser.fz-juelich.de/record/52636},
}