%0 Journal Article
%A Lagarde, F.
%A Pütz, T.
%A Dressel, J.
%A Führ, F.
%T Simultaneous Determination of Ethidimuron, Methabenzthiazuron, and Their Two Major Degradation Products in Soil
%J Journal of agricultural and food chemistry
%V 54
%@ 0021-8561
%C Washington, DC [u.a.]
%I American Chemical Society (ACS)
%M PreJuSER-52843
%P 7450 - 7459
%D 2006
%Z Record converted from VDB: 12.11.2012
%X An analytical method has been developed for the quantification of two herbicides (ethidimuron and methabenzthiazuron) and their two main soil derivatives. This method involves fluidized-bed extraction (FBE) prior to cleanup and analysis by reverse-phase liquid chromatography with UV detection at 282 nm. FBE conditions were established to provide efficient extraction without degradation of the four analytes. (14)C-labeled compounds were used for the optimization of extraction and purification steps and for the determination of related efficiencies. Extraction was optimal using a fexIKA extractor operating at 110 degrees C for three cycles (total time = 95 min) with 75 g of soil and 150 mL of a 60:40 v/v acetone/water mixture. Extracts were further purified on a 500 mg silica SPE cartridge. Separation was performed on a C18 Purosphere column (250 mm x 4 mm i.d.), at 0.8 mL min(-1) and 30 degrees C with an elution gradient made up of phosphoric acid aqueous solution (pH 2.2) and acetonitrile. Calibration curves were found to be linear in the 0.5-50 mg L(-1) concentration range. Besides freshly spiked soil samples, method validation included the analysis of samples with aged residues. Recovery values, determined from spiked samples, were close to 100%. Limits of detection ranged between 2 and 3 microg kg(-1) of dry soil and limits of quantification between 8 and 10 microg kg(-1) of dry soil. An attempt to improve these performances by using fluorescence detection following postcolumn derivatization by orthophthalaldehyde-mercaptoethanol reagent was unsuccessful.
%K Benzothiazoles: analysis
%K Benzothiazoles: chemistry
%K Chromatography, High Pressure Liquid: methods
%K Herbicides: analysis
%K Methylurea Compounds: analysis
%K Methylurea Compounds: chemistry
%K Photolysis
%K Sensitivity and Specificity
%K Soil: analysis
%K Solutions
%K Benzothiazoles (NLM Chemicals)
%K Herbicides (NLM Chemicals)
%K Methylurea Compounds (NLM Chemicals)
%K Soil (NLM Chemicals)
%K Solutions (NLM Chemicals)
%K ethidimuron (NLM Chemicals)
%K methabenzthiazuron (NLM Chemicals)
%K J (WoSType)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:17002407
%U <Go to ISI:>//WOS:000240795400007
%R 10.1021/jf061042v
%U https://juser.fz-juelich.de/record/52843