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@ARTICLE{vandenBosch:52910,
author = {van den Bosch, I. and Lambot, S. and Acheroy, M. and
Huynen, I. and Druyts, P.},
title = {{A}ccurate and efficient modeling of monostatic {GPR}
signal of dielectric targets buried in stratified media},
journal = {Journal of electromagnetic waves and applications},
volume = {20},
issn = {0920-5071},
address = {Zeist},
publisher = {VSP},
reportid = {PreJuSER-52910},
pages = {283 - 290},
year = {2006},
note = {Record converted from VDB: 12.11.2012},
abstract = {A new kind of extracellular polysaccharide (EPS) from
Pseudomonas fluorescens PGM37 was obtained and culture media
was optimized using the statistical methods single factor
experiments and response surface methodology (RSM) design.
As a result, the optimum cultivation conditions initial pH
value, medium volume, inoculum size, temperature, and
rotation speed were 7.5, 100 mL/250 mL, $5 \%,$ 28 °C,
and 180 rpm, respectively. The optimized media: sucrose
36.23 g L(-1), yeast extract 3.32 g L(-1), sodium
chloride 1.13 g L(-1), and calcium chloride
0.20 g L(-1). The maximum predicted yield of EPS was
10.1163 g L(-1) under these conditions. The validation
data was 10.012 g L(-1), which could strongly confirm the
correlation between the experimental and theoretical values.
Gas Chromatography analysis revealed that the polymer was
made up of mannose and glucose in the ratio of 1:1. Infrared
spectroscopy showed that the polysaccharide had
β-D-pyranoid configuration and contained no other
substituent. Graded by different multiples of alcohol after
specific degradation by enzyme and then detected by
LC-ESI-MS, the EPS structure was β-D-Glcp-(1,
4)-β-D-Manp-(1, 4)-β-D-Glcp-(1, 4)-β-D-Manp. The moisture
retention ability of the EPS was found to be superior to
glycerol and only a little inferior to hyaluronic acid (HA),
which presented potential application value in cosmetics and
clinical medicine fields.},
keywords = {J (WoSType)},
cin = {ICG-IV},
ddc = {530},
cid = {I:(DE-Juel1)VDB50},
pnm = {Terrestrische Umwelt},
pid = {G:(DE-Juel1)FUEK407},
shelfmark = {Engineering, Electrical $\&$ Electronic / Physics, Applied
/ Physics, Mathematical},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:23104645},
UT = {WOS:000236237500001},
doi = {10.1163/156939306775701704},
url = {https://juser.fz-juelich.de/record/52910},
}