001     52910
005     20180211165604.0
024 7 _ |2 pmid
|a pmid:23104645
024 7 _ |2 DOI
|a 10.1163/156939306775701704
024 7 _ |2 WOS
|a WOS:000236237500001
037 _ _ |a PreJuSER-52910
041 _ _ |a ENG
082 _ _ |a 530
084 _ _ |2 WoS
|a Engineering, Electrical & Electronic
084 _ _ |2 WoS
|a Physics, Applied
084 _ _ |2 WoS
|a Physics, Mathematical
100 1 _ |a van den Bosch, I.
|b 0
|0 P:(DE-HGF)0
245 _ _ |a Accurate and efficient modeling of monostatic GPR signal of dielectric targets buried in stratified media
260 _ _ |a Zeist
|b VSP
|c 2006
300 _ _ |a 283 - 290
336 7 _ |a Journal Article
|0 PUB:(DE-HGF)16
|2 PUB:(DE-HGF)
336 7 _ |a Output Types/Journal article
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336 7 _ |a Journal Article
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336 7 _ |a ARTICLE
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336 7 _ |a JOURNAL_ARTICLE
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336 7 _ |a article
|2 DRIVER
440 _ 0 |a Journal of Electromagnetic Waves and Applications
|x 0920-5071
|0 15823
|v 20
500 _ _ |a Record converted from VDB: 12.11.2012
520 _ _ |a A new kind of extracellular polysaccharide (EPS) from Pseudomonas fluorescens PGM37 was obtained and culture media was optimized using the statistical methods single factor experiments and response surface methodology (RSM) design. As a result, the optimum cultivation conditions initial pH value, medium volume, inoculum size, temperature, and rotation speed were 7.5, 100 mL/250 mL, 5 %, 28 °C, and 180 rpm, respectively. The optimized media: sucrose 36.23 g L(-1), yeast extract 3.32 g L(-1), sodium chloride 1.13 g L(-1), and calcium chloride 0.20 g L(-1). The maximum predicted yield of EPS was 10.1163 g L(-1) under these conditions. The validation data was 10.012 g L(-1), which could strongly confirm the correlation between the experimental and theoretical values. Gas Chromatography analysis revealed that the polymer was made up of mannose and glucose in the ratio of 1:1. Infrared spectroscopy showed that the polysaccharide had β-D-pyranoid configuration and contained no other substituent. Graded by different multiples of alcohol after specific degradation by enzyme and then detected by LC-ESI-MS, the EPS structure was β-D-Glcp-(1, 4)-β-D-Manp-(1, 4)-β-D-Glcp-(1, 4)-β-D-Manp. The moisture retention ability of the EPS was found to be superior to glycerol and only a little inferior to hyaluronic acid (HA), which presented potential application value in cosmetics and clinical medicine fields.
536 _ _ |a Terrestrische Umwelt
|c P24
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588 _ _ |a Dataset connected to Web of Science, Pubmed
650 _ 7 |a J
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700 1 _ |a Lambot, S.
|b 1
|u FZJ
|0 P:(DE-Juel1)VDB54976
700 1 _ |a Acheroy, M.
|b 2
|0 P:(DE-HGF)0
700 1 _ |a Huynen, I.
|b 3
|0 P:(DE-HGF)0
700 1 _ |a Druyts, P.
|b 4
|0 P:(DE-HGF)0
773 _ _ |a 10.1163/156939306775701704
|g Vol. 20, p. 283 - 290
|p 283 - 290
|q 20<283 - 290
|0 PERI:(DE-600)2069364-3
|t Journal of electromagnetic waves and applications
|v 20
|y 2006
|x 0920-5071
856 7 _ |u http://dx.doi.org/10.1163/156939306775701704
909 C O |o oai:juser.fz-juelich.de:52910
|p VDB
913 1 _ |k P24
|v Terrestrische Umwelt
|l Terrestrische Umwelt
|b Erde und Umwelt
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|x 0
914 1 _ |y 2006
915 _ _ |0 StatID:(DE-HGF)0010
|a JCR/ISI refereed
920 1 _ |k ICG-IV
|l Agrosphäre
|d 31.12.2006
|g ICG
|0 I:(DE-Juel1)VDB50
|x 0
970 _ _ |a VDB:(DE-Juel1)83240
980 _ _ |a VDB
980 _ _ |a ConvertedRecord
980 _ _ |a journal
980 _ _ |a I:(DE-Juel1)IBG-3-20101118
980 _ _ |a UNRESTRICTED
981 _ _ |a I:(DE-Juel1)IBG-3-20101118


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