000005317 001__ 5317
000005317 005__ 20200402205630.0
000005317 0247_ $$2pmid$$apmid:19267872
000005317 0247_ $$2DOI$$a10.1111/j.1751-1097.2009.00539.x
000005317 0247_ $$2WOS$$aWOS:000266034900009
000005317 037__ $$aPreJuSER-5317
000005317 041__ $$aeng
000005317 082__ $$a570
000005317 084__ $$2WoS$$aBiochemistry & Molecular Biology
000005317 084__ $$2WoS$$aBiophysics
000005317 1001_ $$0P:(DE-Juel1)VDB86778$$aBalem, F.$$b0$$uFZJ
000005317 245__ $$aAdditive Effects of Chlorin E6 and Metal Ion Binding on the Thermal Stability of Rhodopsin in Vitro
000005317 260__ $$aMalden, Mass.$$bWiley-Blackwell$$c2009
000005317 300__ $$a471 - 478
000005317 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article
000005317 3367_ $$2DataCite$$aOutput Types/Journal article
000005317 3367_ $$00$$2EndNote$$aJournal Article
000005317 3367_ $$2BibTeX$$aARTICLE
000005317 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000005317 3367_ $$2DRIVER$$aarticle
000005317 440_0 $$015281$$aPhotochemistry and Photobiology$$v85$$x0031-8655$$y2
000005317 500__ $$aRecord converted from VDB: 12.11.2012
000005317 520__ $$aZinc (Zn(2+)) deficiency causes retinal dysfunctions such as night blindness and neurodegeneration. Because Zn(2+) binds directly to the photoreceptor rhodopsin and alters its stability, the stabilization of rhodopsin may be key to prevention and treatment of retinal dysfunctions. In this paper, we investigated if not only trace metals but also other nutrients may stabilize rhodopsin structure in vitro. Detailed studies of the thermal stability of secondary and tertiary structure of rhodopsin in the presence and absence of the chlorophyll derivative chlorin e6 alone and together with bivalent metal ions Zn(2+), Cu(2+), Fe(2+), Ni(2+), Mg(2+)and Mn(2+) over a temperature range 5-100 degrees C were conducted using circular dichroism and fluorescence spectroscopy. When both chlorin e6 and Zn(2+) are present, a pronounced increase in the thermal stability of overall secondary structure content is observed compared to either compound alone. This additive capacity is also noted with Cu(2+), but not when other metal ions and chlorin e6 are combined.
000005317 536__ $$0G:(DE-Juel1)FUEK307$$2G:(DE-HGF)$$aohne FE$$cohne FE$$x0
000005317 588__ $$aDataset connected to Web of Science, Pubmed
000005317 650_2 $$2MeSH$$aAnimals
000005317 650_2 $$2MeSH$$aCattle
000005317 650_2 $$2MeSH$$aCircular Dichroism
000005317 650_2 $$2MeSH$$aIons: chemistry
000005317 650_2 $$2MeSH$$aLight
000005317 650_2 $$2MeSH$$aMetals: chemistry
000005317 650_2 $$2MeSH$$aMetals: metabolism
000005317 650_2 $$2MeSH$$aModels, Molecular
000005317 650_2 $$2MeSH$$aPorphyrins: chemistry
000005317 650_2 $$2MeSH$$aPorphyrins: metabolism
000005317 650_2 $$2MeSH$$aProtein Binding
000005317 650_2 $$2MeSH$$aProtein Denaturation
000005317 650_2 $$2MeSH$$aProtein Structure, Secondary
000005317 650_2 $$2MeSH$$aProtein Structure, Tertiary
000005317 650_2 $$2MeSH$$aRhodopsin: chemistry
000005317 650_2 $$2MeSH$$aRhodopsin: metabolism
000005317 650_2 $$2MeSH$$aTemperature
000005317 650_7 $$00$$2NLM Chemicals$$aIons
000005317 650_7 $$00$$2NLM Chemicals$$aMetals
000005317 650_7 $$00$$2NLM Chemicals$$aPorphyrins
000005317 650_7 $$019660-77-6$$2NLM Chemicals$$achlorin e6
000005317 650_7 $$09009-81-8$$2NLM Chemicals$$aRhodopsin
000005317 650_7 $$2WoSType$$aJ
000005317 7001_ $$0P:(DE-HGF)0$$aYanamala, N.$$b1
000005317 7001_ $$0P:(DE-Juel1)VDB44599$$aKlein-Seetharaman, J.$$b2$$uFZJ
000005317 773__ $$0PERI:(DE-600)2048860-9$$a10.1111/j.1751-1097.2009.00539.x$$gVol. 85, p. 471 - 478$$p471 - 478$$q85<471 - 478$$tPhotochemistry and photobiology$$v85$$x0031-8655$$y2009
000005317 8567_ $$uhttp://dx.doi.org/10.1111/j.1751-1097.2009.00539.x
000005317 909CO $$ooai:juser.fz-juelich.de:5317$$pVDB
000005317 9131_ $$0G:(DE-Juel1)FUEK307$$bohne FE$$kohne FE$$lohne FE$$vohne FE$$x0
000005317 9141_ $$y2009
000005317 915__ $$0StatID:(DE-HGF)0010$$aJCR/ISI refereed
000005317 9201_ $$0I:(DE-Juel1)ISB-2-20090406$$d31.12.2010$$gISB$$kISB-2$$lMolekulare Biophysik$$x0
000005317 970__ $$aVDB:(DE-Juel1)112686
000005317 980__ $$aVDB
000005317 980__ $$aConvertedRecord
000005317 980__ $$ajournal
000005317 980__ $$aI:(DE-Juel1)ICS-6-20110106
000005317 980__ $$aUNRESTRICTED
000005317 981__ $$aI:(DE-Juel1)IBI-7-20200312
000005317 981__ $$aI:(DE-Juel1)ICS-6-20110106
000005317 981__ $$aI:(DE-Juel1)ISB-2-20090406