TY  - JOUR
AU  - Balem, F.
AU  - Yanamala, N.
AU  - Klein-Seetharaman, J.
TI  - Additive Effects of Chlorin E6 and Metal Ion Binding on the Thermal Stability of Rhodopsin in Vitro
JO  - Photochemistry and photobiology
VL  - 85
SN  - 0031-8655
CY  - Malden, Mass.
PB  - Wiley-Blackwell
M1  - PreJuSER-5317
SP  - 471 - 478
PY  - 2009
N1  - Record converted from VDB: 12.11.2012
AB  - Zinc (Zn(2+)) deficiency causes retinal dysfunctions such as night blindness and neurodegeneration. Because Zn(2+) binds directly to the photoreceptor rhodopsin and alters its stability, the stabilization of rhodopsin may be key to prevention and treatment of retinal dysfunctions. In this paper, we investigated if not only trace metals but also other nutrients may stabilize rhodopsin structure in vitro. Detailed studies of the thermal stability of secondary and tertiary structure of rhodopsin in the presence and absence of the chlorophyll derivative chlorin e6 alone and together with bivalent metal ions Zn(2+), Cu(2+), Fe(2+), Ni(2+), Mg(2+)and Mn(2+) over a temperature range 5-100 degrees C were conducted using circular dichroism and fluorescence spectroscopy. When both chlorin e6 and Zn(2+) are present, a pronounced increase in the thermal stability of overall secondary structure content is observed compared to either compound alone. This additive capacity is also noted with Cu(2+), but not when other metal ions and chlorin e6 are combined.
KW  - Animals
KW  - Cattle
KW  - Circular Dichroism
KW  - Ions: chemistry
KW  - Light
KW  - Metals: chemistry
KW  - Metals: metabolism
KW  - Models, Molecular
KW  - Porphyrins: chemistry
KW  - Porphyrins: metabolism
KW  - Protein Binding
KW  - Protein Denaturation
KW  - Protein Structure, Secondary
KW  - Protein Structure, Tertiary
KW  - Rhodopsin: chemistry
KW  - Rhodopsin: metabolism
KW  - Temperature
KW  - Ions (NLM Chemicals)
KW  - Metals (NLM Chemicals)
KW  - Porphyrins (NLM Chemicals)
KW  - chlorin e6 (NLM Chemicals)
KW  - Rhodopsin (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:19267872
UR  - <Go to ISI:>//WOS:000266034900009
DO  - DOI:10.1111/j.1751-1097.2009.00539.x
UR  - https://juser.fz-juelich.de/record/5317
ER  -