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@ARTICLE{Balem:5317,
      author       = {Balem, F. and Yanamala, N. and Klein-Seetharaman, J.},
      title        = {{A}dditive {E}ffects of {C}hlorin {E}6 and {M}etal {I}on
                      {B}inding on the {T}hermal {S}tability of {R}hodopsin in
                      {V}itro},
      journal      = {Photochemistry and photobiology},
      volume       = {85},
      issn         = {0031-8655},
      address      = {Malden, Mass.},
      publisher    = {Wiley-Blackwell},
      reportid     = {PreJuSER-5317},
      pages        = {471 - 478},
      year         = {2009},
      note         = {Record converted from VDB: 12.11.2012},
      abstract     = {Zinc (Zn(2+)) deficiency causes retinal dysfunctions such
                      as night blindness and neurodegeneration. Because Zn(2+)
                      binds directly to the photoreceptor rhodopsin and alters its
                      stability, the stabilization of rhodopsin may be key to
                      prevention and treatment of retinal dysfunctions. In this
                      paper, we investigated if not only trace metals but also
                      other nutrients may stabilize rhodopsin structure in vitro.
                      Detailed studies of the thermal stability of secondary and
                      tertiary structure of rhodopsin in the presence and absence
                      of the chlorophyll derivative chlorin e6 alone and together
                      with bivalent metal ions Zn(2+), Cu(2+), Fe(2+), Ni(2+),
                      Mg(2+)and Mn(2+) over a temperature range 5-100 degrees C
                      were conducted using circular dichroism and fluorescence
                      spectroscopy. When both chlorin e6 and Zn(2+) are present, a
                      pronounced increase in the thermal stability of overall
                      secondary structure content is observed compared to either
                      compound alone. This additive capacity is also noted with
                      Cu(2+), but not when other metal ions and chlorin e6 are
                      combined.},
      keywords     = {Animals / Cattle / Circular Dichroism / Ions: chemistry /
                      Light / Metals: chemistry / Metals: metabolism / Models,
                      Molecular / Porphyrins: chemistry / Porphyrins: metabolism /
                      Protein Binding / Protein Denaturation / Protein Structure,
                      Secondary / Protein Structure, Tertiary / Rhodopsin:
                      chemistry / Rhodopsin: metabolism / Temperature / Ions (NLM
                      Chemicals) / Metals (NLM Chemicals) / Porphyrins (NLM
                      Chemicals) / chlorin e6 (NLM Chemicals) / Rhodopsin (NLM
                      Chemicals) / J (WoSType)},
      cin          = {ISB-2},
      ddc          = {570},
      cid          = {I:(DE-Juel1)ISB-2-20090406},
      pnm          = {ohne FE},
      pid          = {G:(DE-Juel1)FUEK307},
      shelfmark    = {Biochemistry $\&$ Molecular Biology / Biophysics},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:19267872},
      UT           = {WOS:000266034900009},
      doi          = {10.1111/j.1751-1097.2009.00539.x},
      url          = {https://juser.fz-juelich.de/record/5317},
}