001     53356
005     20180211180541.0
024 7 _ |a pmid:23104645
|2 pmid
024 7 _ |a 10.1163/156939604777303235
|2 DOI
037 _ _ |a PreJuSER-53356
041 _ _ |a ENG
082 _ _ |a 510
100 1 _ |a Suciu, N.
|b 0
|0 P:(DE-HGF)0
245 _ _ |a Numerical Modeling of Large Scale Transport of Comtaminant Solutes Using the Global Random Walk Algorithm
260 _ _ |a Berlin [u.a.]
|b de Gruyter
|c 2004
300 _ _ |a 153 - 177
336 7 _ |a Journal Article
|0 PUB:(DE-HGF)16
|2 PUB:(DE-HGF)
336 7 _ |a Output Types/Journal article
|2 DataCite
336 7 _ |a Journal Article
|0 0
|2 EndNote
336 7 _ |a ARTICLE
|2 BibTeX
336 7 _ |a JOURNAL_ARTICLE
|2 ORCID
336 7 _ |a article
|2 DRIVER
440 _ 0 |a Monte Carlo Methods and Applications
|x 0929-9629
|0 16001
|y 2
|v 10
500 _ _ |a Record converted from VDB: 12.11.2012
520 _ _ |a A new kind of extracellular polysaccharide (EPS) from Pseudomonas fluorescens PGM37 was obtained and culture media was optimized using the statistical methods single factor experiments and response surface methodology (RSM) design. As a result, the optimum cultivation conditions initial pH value, medium volume, inoculum size, temperature, and rotation speed were 7.5, 100 mL/250 mL, 5 %, 28 °C, and 180 rpm, respectively. The optimized media: sucrose 36.23 g L(-1), yeast extract 3.32 g L(-1), sodium chloride 1.13 g L(-1), and calcium chloride 0.20 g L(-1). The maximum predicted yield of EPS was 10.1163 g L(-1) under these conditions. The validation data was 10.012 g L(-1), which could strongly confirm the correlation between the experimental and theoretical values. Gas Chromatography analysis revealed that the polymer was made up of mannose and glucose in the ratio of 1:1. Infrared spectroscopy showed that the polysaccharide had β-D-pyranoid configuration and contained no other substituent. Graded by different multiples of alcohol after specific degradation by enzyme and then detected by LC-ESI-MS, the EPS structure was β-D-Glcp-(1, 4)-β-D-Manp-(1, 4)-β-D-Glcp-(1, 4)-β-D-Manp. The moisture retention ability of the EPS was found to be superior to glycerol and only a little inferior to hyaluronic acid (HA), which presented potential application value in cosmetics and clinical medicine fields.
536 _ _ |a Chemie und Dynamik der Geo-Biosphäre
|c U01
|2 G:(DE-HGF)
|0 G:(DE-Juel1)FUEK257
|x 0
588 _ _ |a Dataset connected to Pubmed
700 1 _ |a Vamos, C.
|b 1
|0 P:(DE-HGF)0
700 1 _ |a Hardelauf, H.
|b 2
|u FZJ
|0 P:(DE-Juel1)129466
700 1 _ |a Vanderborght, J.
|b 3
|u FZJ
|0 P:(DE-Juel1)129548
700 1 _ |a Vereecken, H.
|b 4
|u FZJ
|0 P:(DE-Juel1)129549
773 _ _ |a 10.1163/156939604777303235
|g Vol. 10, p. 153 - 177
|p 153 - 177
|q 10<153 - 177
|0 PERI:(DE-600)2043509-5
|t Monte Carlo methods and applications
|v 10
|y 2004
|x 0929-9629
856 7 _ |u http://dx.doi.org/10.1163/156939604777303235
909 C O |o oai:juser.fz-juelich.de:53356
|p VDB
913 1 _ |k U01
|v Chemie und Dynamik der Geo-Biosphäre
|l Chemie und Dynamik der Geo-Biosphäre
|b Environment (Umwelt)
|0 G:(DE-Juel1)FUEK257
|x 0
914 1 _ |a Nachtrag
|y 2004
915 _ _ |0 StatID:(DE-HGF)0020
|a No peer review
920 1 _ |k ICG-IV
|l Agrosphäre
|d 31.12.2006
|g ICG
|0 I:(DE-Juel1)VDB50
|x 0
970 _ _ |a VDB:(DE-Juel1)83844
980 _ _ |a VDB
980 _ _ |a ConvertedRecord
980 _ _ |a journal
980 _ _ |a I:(DE-Juel1)IBG-3-20101118
980 _ _ |a UNRESTRICTED
981 _ _ |a I:(DE-Juel1)IBG-3-20101118


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