TY  - JOUR
AU  - Ferrieri, A.P.
AU  - Thorpe, M. R.
AU  - Ferrieri, R. A.
TI  - Stimulating natural defenses in poplar clones (OP-367) increases plant metabolism of carbon tetrachloride
JO  - International journal of phytoremediation
VL  - 8
SN  - 1522-6514
CY  - Philadelphia, Pa.
PB  - Taylor & Francis
M1  - PreJuSER-53637
SP  - 233 - 243
PY  - 2006
N1  - Record converted from VDB: 12.11.2012
AB  - Groundwater contamination by carbon tetrachloride (CCl4) presents a health risk as a potential carcinogen and pollutant that is capable of depleting the ozone layer. Although use of poplar trees in a phytoremediation capacity has proven to be cost effective for cleaning contaminated sites, minimizing leaf emission of volatile contaminants remains a pressing issue. We hypothesized that recently fixed carbon plays a key role in CCl4 metabolism in planta yielding nonvolatile trichloroacetic acid (TCA) and that the extent of this metabolism can be altered by heightening plant defenses. Labeling intact leaves with (11)CO2 (t 1/2 20.4 m) can test this hypothesis, because the extremely short half-life of the tracer reflects only those processes involving recently fixed carbon. Using radio-HPLC analysis, we observed [(11)C]TCA from leaf extract from poplar clones (OP-367) whose roots were exposed to a saturated solution of CCl4 (520 ppm). Autoradiography of [(11)C]photosynthate showed increased leaf export and partitioning to the apex within 24 h of CCl4 exposure, suggesting that changes in plant metabolism and partitioning of recently fixed carbon occur rapidly. Additionally, leaf CCl4 emissions were highest in the morning, when carbon pools are low, suggesting a link between contaminant metabolism and leaf carbon utilization. Further, treatment with methyljasmonate, a plant hormone implicated in defense signal transduction, reduced leaf CCl4 emissions two-fold due to the increased formation of TCA.
KW  - Acetic Acids: pharmacology
KW  - Carbon Radioisotopes: analysis
KW  - Carbon Tetrachloride: metabolism
KW  - Chromatography, High Pressure Liquid
KW  - Cyclopentanes: pharmacology
KW  - Oxylipins
KW  - Photosynthesis: drug effects
KW  - Plant Growth Regulators: pharmacology
KW  - Plant Leaves: metabolism
KW  - Populus: drug effects
KW  - Populus: metabolism
KW  - Trichloroacetic Acid: metabolism
KW  - Acetic Acids (NLM Chemicals)
KW  - Carbon Radioisotopes (NLM Chemicals)
KW  - Cyclopentanes (NLM Chemicals)
KW  - Oxylipins (NLM Chemicals)
KW  - Plant Growth Regulators (NLM Chemicals)
KW  - methyl jasmonate (NLM Chemicals)
KW  - Carbon Tetrachloride (NLM Chemicals)
KW  - Trichloroacetic Acid (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:17120527
UR  - <Go to ISI:>//WOS:000240701200004
DO  - DOI:10.1080/15226510600846780
UR  - https://juser.fz-juelich.de/record/53637
ER  -