%0 Journal Article
%A Weiergräber, O. H.
%A Senin, I. I.
%A Zernii, E.Y.
%A Churumova, V. A.
%A Kovaleva, N. A.
%A Nazipova, A. A.
%A Permyakov, S.E.
%A Permyakov, E.A.
%A Philippov, P.P.
%A Granzin, J.
%A Koch, K. W.
%T Tuning of a neuronal calcium sensor
%J The journal of biological chemistry
%V 281
%@ 0021-9258
%C Bethesda, Md.
%I Soc.
%M PreJuSER-54334
%P 37594 - 37602
%D 2006
%Z Record converted from VDB: 12.11.2012
%X Recoverin is a Ca(2+)-regulated signal transduction modulator expressed in the vertebrate retina that has been implicated in visual adaptation. An intriguing feature of recoverin is a cluster of charged residues at its C terminus, the functional significance of which is largely unclear. To elucidate the impact of this segment on recoverin structure and function, we have investigated a mutant lacking the C-terminal 12 amino acids. Whereas in myristoylated recoverin the truncation causes an overall decrease in Ca(2+) sensitivity, results for the non-myristoylated mutant indicate that the truncation primarily affects the high affinity EF-hand 3. The three-dimensional structure of the mutant has been determined by x-ray crystallography. In addition to significant changes in average coordinates compared with wild-type recoverin, the structure provides strong indication of increased conformational flexibility, particularly in the C-terminal domain. Based on these observations, we propose a novel role of the C-terminal segment of recoverin as an internal modulator of Ca(2+) sensitivity.
%K Animals
%K Base Sequence
%K Calcium Signaling: physiology
%K Cattle
%K Crystallography, X-Ray
%K DNA Primers: genetics
%K Kinetics
%K Models, Molecular
%K Mutagenesis, Site-Directed
%K Protein Structure, Quaternary
%K Recombinant Proteins: chemistry
%K Recombinant Proteins: genetics
%K Recombinant Proteins: metabolism
%K Recoverin: chemistry
%K Recoverin: genetics
%K Recoverin: metabolism
%K Rod Cell Outer Segment: metabolism
%K Sequence Deletion
%K Surface Plasmon Resonance
%K DNA Primers (NLM Chemicals)
%K RCV1 protein, Bos taurus (NLM Chemicals)
%K Recombinant Proteins (NLM Chemicals)
%K Recoverin (NLM Chemicals)
%K J (WoSType)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:17015448
%U <Go to ISI:>//WOS:000242477100036
%R 10.1074/jbc.M603700200
%U https://juser.fz-juelich.de/record/54334