TY  - JOUR
AU  - Weiergräber, O. H.
AU  - Senin, I. I.
AU  - Zernii, E.Y.
AU  - Churumova, V. A.
AU  - Kovaleva, N. A.
AU  - Nazipova, A. A.
AU  - Permyakov, S.E.
AU  - Permyakov, E.A.
AU  - Philippov, P.P.
AU  - Granzin, J.
AU  - Koch, K. W.
TI  - Tuning of a neuronal calcium sensor
JO  - The journal of biological chemistry
VL  - 281
SN  - 0021-9258
CY  - Bethesda, Md.
PB  - Soc.
M1  - PreJuSER-54334
SP  - 37594 - 37602
PY  - 2006
N1  - Record converted from VDB: 12.11.2012
AB  - Recoverin is a Ca(2+)-regulated signal transduction modulator expressed in the vertebrate retina that has been implicated in visual adaptation. An intriguing feature of recoverin is a cluster of charged residues at its C terminus, the functional significance of which is largely unclear. To elucidate the impact of this segment on recoverin structure and function, we have investigated a mutant lacking the C-terminal 12 amino acids. Whereas in myristoylated recoverin the truncation causes an overall decrease in Ca(2+) sensitivity, results for the non-myristoylated mutant indicate that the truncation primarily affects the high affinity EF-hand 3. The three-dimensional structure of the mutant has been determined by x-ray crystallography. In addition to significant changes in average coordinates compared with wild-type recoverin, the structure provides strong indication of increased conformational flexibility, particularly in the C-terminal domain. Based on these observations, we propose a novel role of the C-terminal segment of recoverin as an internal modulator of Ca(2+) sensitivity.
KW  - Animals
KW  - Base Sequence
KW  - Calcium Signaling: physiology
KW  - Cattle
KW  - Crystallography, X-Ray
KW  - DNA Primers: genetics
KW  - Kinetics
KW  - Models, Molecular
KW  - Mutagenesis, Site-Directed
KW  - Protein Structure, Quaternary
KW  - Recombinant Proteins: chemistry
KW  - Recombinant Proteins: genetics
KW  - Recombinant Proteins: metabolism
KW  - Recoverin: chemistry
KW  - Recoverin: genetics
KW  - Recoverin: metabolism
KW  - Rod Cell Outer Segment: metabolism
KW  - Sequence Deletion
KW  - Surface Plasmon Resonance
KW  - DNA Primers (NLM Chemicals)
KW  - RCV1 protein, Bos taurus (NLM Chemicals)
KW  - Recombinant Proteins (NLM Chemicals)
KW  - Recoverin (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:17015448
UR  - <Go to ISI:>//WOS:000242477100036
DO  - DOI:10.1074/jbc.M603700200
UR  - https://juser.fz-juelich.de/record/54334
ER  -