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@ARTICLE{Winkler:54926,
author = {Winkler, R. G. and Keller, S. and Rädler, J. O.},
title = {{I}ntramolecular dynamics of linear macromolecules by
fluorescence correlation spectroscopy},
journal = {Physical review / E},
volume = {73},
number = {4},
issn = {1539-3755},
address = {College Park, Md.},
publisher = {APS},
reportid = {PreJuSER-54926},
pages = {041919},
year = {2006},
note = {Record converted from VDB: 12.11.2012},
abstract = {A theoretical description of the dynamics of DNA molecules
and actin filaments in solution as measured experimentally
by fluorescence correlation spectroscopy is provided and
compared to recent experimental results. Particular
attention is paid to the contribution of the intramolecular
dynamics to the fluorescence correlation function. Using a
semiflexible chain model, a theoretical expression is
presented for the fluorescence correlation spectroscopy
correlation function. The dependence of this function on
various model parameters, such as chain length, persistence
length, and fluorescence label density, is discussed. Our
investigations show that the intramolecular dynamics
provides a significant contribution or even dominates the
correlation function as soon as the longest intramolecular
relaxation time significantly exceeds the shortest
experimentally accessible time. Correspondingly, the shape
of the correlation function changes considerably.
Approximate analytical expressions are provided, which are
in qualitative agreement with the exact theoretical
solutions as well as experimental results, for both DNA and
actin filaments. Our approach is in agreement with the
predictions of the Zimm model, in the limit of very flexible
polymers, as well as the predictions of semiflexible polymer
models with respect to the intramolecular dynamics in
solution.},
keywords = {J (WoSType)},
cin = {IFF-TH-II},
ddc = {530},
cid = {I:(DE-Juel1)VDB31},
pnm = {Kondensierte Materie},
pid = {G:(DE-Juel1)FUEK414},
shelfmark = {Physics, Fluids $\&$ Plasmas / Physics, Mathematical},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000237146400076},
doi = {10.1103/PhysRevE.73.041919},
url = {https://juser.fz-juelich.de/record/54926},
}