TY  - JOUR
AU  - Haenel, K.
AU  - Stangler, T.
AU  - Stoldt, M.
AU  - Willbold, D.
TI  - Solution structure of the X4 protein coded by the SARS related coronavirus reveals an immunoglobulin like fold and suggests a binding activity to integrin I domains
JO  - Journal of biomedical science
VL  - 13
SN  - 1021-7770
CY  - London
PB  - BioMed Central
M1  - PreJuSER-55020
SP  - 281 - 293
PY  - 2006
N1  - Record converted from VDB: 12.11.2012
AB  - The SARS related Coronavirus genome contains a variety of novel accessory genes. One of these, called ORF7a or ORF8, code for a protein, known as 7a, U122 or X4. We set out to determine the three-dimensional structure of the soluble ectodomain of this type-I transmembrane protein by nuclear magnetic resonance spectroscopy. The fold of the protein is the first member of a further variation of the immunoglobulin like beta-sandwich fold. Because X4 does not reveal significant sequence homologies to proteins in the data bases, we carried out a structure based similarity search for proteins with known function. High structural similarity to Dl domains of ICAM-1 and ICAM-2, and common features in amino acid sequence between X4 and ICAM-1, suggest X4 to possess binding activity for the alpha(L) integrin I domain of LFA-1. Further, based on this structure based prediction, potential functions of X4 in virus replication and pathogenesis are discussed.
KW  - Amino Acid Sequence
KW  - Antigens, CD: chemistry
KW  - Cell Adhesion Molecules: chemistry
KW  - Cloning, Molecular
KW  - Humans
KW  - Immunoglobulins: chemistry
KW  - Integrins: chemistry
KW  - Intercellular Adhesion Molecule-1: chemistry
KW  - Interleukin-1: chemistry
KW  - Lymphocyte Function-Associated Antigen-1: chemistry
KW  - Models, Molecular
KW  - Molecular Sequence Data
KW  - Open Reading Frames
KW  - Protein Structure, Secondary
KW  - Protein Structure, Tertiary
KW  - SARS Virus: chemistry
KW  - Viral Matrix Proteins: chemistry
KW  - Viral Proteins: chemistry
KW  - Antigens, CD (NLM Chemicals)
KW  - Cell Adhesion Molecules (NLM Chemicals)
KW  - Immunoglobulins (NLM Chemicals)
KW  - Integrins (NLM Chemicals)
KW  - Interleukin-1 (NLM Chemicals)
KW  - Lymphocyte Function-Associated Antigen-1 (NLM Chemicals)
KW  - Viral Matrix Proteins (NLM Chemicals)
KW  - Viral Proteins (NLM Chemicals)
KW  - sars7a protein, SARS virus (NLM Chemicals)
KW  - Intercellular Adhesion Molecule-1 (NLM Chemicals)
KW  - ICAM2 protein, human (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:16328780
UR  - <Go to ISI:>//WOS:000237502100001
DO  - DOI:10.1007/s11373-005-9043-9
UR  - https://juser.fz-juelich.de/record/55020
ER  -