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@ARTICLE{Psurek:55022,
      author       = {Psurek, A. and Feuerstein, S.E. and Willbold, D. and
                      Scriba, G. K. E.},
      title        = {{N}onaqueous versus aqueous capillary electrophoresis of
                      alpha-helical polypeptides: {E}ffect of secondary structure
                      on separation selectivity},
      journal      = {Electrophoresis},
      volume       = {27},
      issn         = {0173-0835},
      address      = {Weinheim},
      publisher    = {Wiley-Blackwell},
      reportid     = {PreJuSER-55022},
      pages        = {1768 - 1775},
      year         = {2006},
      note         = {Record converted from VDB: 12.11.2012},
      abstract     = {The CE separation of alpha-helical polypeptides composed of
                      14-31 amino acid residues has been investigated using
                      aqueous and nonaqueous BGEs. The running buffers were
                      optimized with respect to pH. Generally, higher separation
                      selectivities were observed in nonaqueous electrolytes. This
                      may be explained by a change in the secondary structure when
                      changing from water to organic solvents. Circular dichroism
                      spectra revealed a significant increase in helical
                      structures in methanol-based buffers compared to aqueous
                      buffers. This change in secondary structure of the
                      polypeptides contributed primarily to the different
                      separation selectivity observed in aqueous CE and NACE. For
                      small oligopeptides of two to five amino acid residues no
                      significant effect of the solvent was observed in some cases
                      while in other cases a reversal of the migration order
                      occurred when changing from aqueous to nonaqueous buffers.
                      As these peptides cannot adopt secondary structures the
                      effect may be attributed to a shift of the pKa values in
                      organic solvents compared to water.},
      keywords     = {Amino Acid Sequence / Circular Dichroism / Electrophoresis,
                      Capillary: methods / Molecular Sequence Data / Peptides:
                      chemistry / Peptides: isolation $\&$ purification / Protein
                      Structure, Secondary / Water: chemistry / Peptides (NLM
                      Chemicals) / Water (NLM Chemicals) / J (WoSType)},
      cin          = {IBI-2},
      ddc          = {570},
      cid          = {I:(DE-Juel1)VDB58},
      pnm          = {Funktion und Dysfunktion des Nervensystems},
      pid          = {G:(DE-Juel1)FUEK409},
      shelfmark    = {Biochemical Research Methods / Chemistry, Analytical},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:16645940},
      UT           = {WOS:000237685600011},
      doi          = {10.1002/elps.200500673},
      url          = {https://juser.fz-juelich.de/record/55022},
}