TY  - JOUR
AU  - Cremer, C.M.
AU  - Palomero-Gallagher, N.
AU  - Bidmon, H.-J.
AU  - Schleicher, A.
AU  - Speckmann, E.-J.
AU  - Zilles, K.
TI  - Pentylenetetrazole-induced seizures affect binding site densities for GABA, glutamate and adenosine receptors in the rat brain
JO  - Neuroscience
VL  - 163
SN  - 0306-4522
CY  - Amsterdam [u.a.]
PB  - Elsevier Science
M1  - PreJuSER-5513
SP  - 490 - 499
PY  - 2009
N1  - Record converted from VDB: 12.11.2012
AB  - Pentylenetetrazole (PTZ) is a convulsant used to model epileptic seizures in rats. In the PTZ-model, altered heat shock protein 27 (HSP-27) expression highlights seizure-affected astrocytes, which play an important role in glutamate and GABA metabolism. This raises the question whether impaired neurotransmitter metabolism leads to an imbalance in neurotransmitter receptor expression. Consequently, we investigated the effects of seizures on the densities of seven different neurotransmitter receptors in rats which were repeatedly treated with PTZ (40 mg/kg) over a period of 14 days. Quantitative in vitro receptor autoradiography was used to measure the regional binding site densities of the glutamate alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA), kainate and N-methyl-D-aspartate (NMDA) receptors, the adenosine receptor type 1 (A(1)), which is part of the system controlling glutamate release, and the gamma-aminobutyric acid (GABA) receptors GABA(A) and GABA(B) as well as the GABA(A)-associated benzodiazepine (BZ) binding sites in each rat. Our results demonstrate altered receptor densities in brain regions of PTZ-treated animals, including the HSP-27 expressing foci (i.e. amygdala, piriform and entorhinal cortex, dentate gyrus). A general decrease of kainate receptor densities was observed together with an increase of NMDA binding sites in the hippocampus, the somatosensory, piriform and the entorhinal cortices. Furthermore, A(1) binding sites were decreased in the amygdala and hippocampal CA1 region (CA1), while BZ binding sites were increased in the dentate gyrus and CA1. Our data demonstrate the impact of PTZ induced seizures on the densities of kainate, NMDA, A(1) and BZ binding sites in epileptic brain. These changes are not restricted to regions showing glial impairment. Thus, an altered balance between different excitatory (NMDA) and modulatory receptors (A(1), BZ binding sites, kainate) shows a much wider regional distribution than that of glial HSP-27 expression, indicating that receptor changes are not following the glial stress responses, but may precede the HSP-27 expression.
KW  - Animals
KW  - Binding Sites: drug effects
KW  - Binding Sites: physiology
KW  - Binding, Competitive: drug effects
KW  - Binding, Competitive: physiology
KW  - Brain: anatomy & histology
KW  - Brain: metabolism
KW  - Brain: physiopathology
KW  - Convulsants: pharmacology
KW  - Disease Models, Animal
KW  - Epilepsy: chemically induced
KW  - Epilepsy: metabolism
KW  - Epilepsy: physiopathology
KW  - HSP27 Heat-Shock Proteins: drug effects
KW  - HSP27 Heat-Shock Proteins: metabolism
KW  - Male
KW  - Neuroglia: metabolism
KW  - Pentylenetetrazole: pharmacology
KW  - Rats
KW  - Rats, Wistar
KW  - Receptor, Adenosine A1: drug effects
KW  - Receptor, Adenosine A1: metabolism
KW  - Receptors, AMPA: drug effects
KW  - Receptors, AMPA: metabolism
KW  - Receptors, GABA: metabolism
KW  - Receptors, GABA-A: drug effects
KW  - Receptors, GABA-A: metabolism
KW  - Receptors, GABA-B: drug effects
KW  - Receptors, GABA-B: metabolism
KW  - Receptors, Glutamate: metabolism
KW  - Receptors, Kainic Acid: drug effects
KW  - Receptors, Kainic Acid: metabolism
KW  - Receptors, N-Methyl-D-Aspartate: drug effects
KW  - Receptors, N-Methyl-D-Aspartate: metabolism
KW  - Receptors, Purinergic P1: metabolism
KW  - Stress, Physiological: physiology
KW  - Synaptic Transmission: drug effects
KW  - Synaptic Transmission: physiology
KW  - Convulsants (NLM Chemicals)
KW  - HSP27 Heat-Shock Proteins (NLM Chemicals)
KW  - Hspb1 protein, rat (NLM Chemicals)
KW  - Receptor, Adenosine A1 (NLM Chemicals)
KW  - Receptors, AMPA (NLM Chemicals)
KW  - Receptors, GABA (NLM Chemicals)
KW  - Receptors, GABA-A (NLM Chemicals)
KW  - Receptors, GABA-B (NLM Chemicals)
KW  - Receptors, Glutamate (NLM Chemicals)
KW  - Receptors, Kainic Acid (NLM Chemicals)
KW  - Receptors, N-Methyl-D-Aspartate (NLM Chemicals)
KW  - Receptors, Purinergic P1 (NLM Chemicals)
KW  - Pentylenetetrazole (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:19345722
UR  - <Go to ISI:>//WOS:000269404600044
DO  - DOI:10.1016/j.neuroscience.2009.03.068
UR  - https://juser.fz-juelich.de/record/5513
ER  -