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000005526 084__ $$2WoS$$aEnvironmental Sciences
000005526 084__ $$2WoS$$aSoil Science
000005526 084__ $$2WoS$$aWater Resources
000005526 1001_ $$0P:(DE-Juel1)VDB940$$aLiebich, J.$$b0$$uFZJ
000005526 245__ $$aDegradation of Diffuse Pesticide Contaminants: Screening for Microbial Potential Using a Functional Gene Microarray
000005526 260__ $$aMadison, Wis.$$bSSSA$$c2009
000005526 300__ $$a703 - 710
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000005526 440_0 $$010301$$aVadose Zone Journal$$v8$$x1539-1663$$y3
000005526 500__ $$aWe would like to thank Hans Gunther Sittardt for assistance during sampling and for providing atrazine contamination data, Dr. Tappe and Prof. Ruttkowski (Aachen University of Applied Sciences) for helpful discussions, and Dr. Canty and Hazel Rochford for their linguistic revisions. The construction of the GeoChip was supported within the framework of the Environmental Stress Pathway Project (ESPP) of the Virtual Institute for Microbial Stress and Survival (http://vimss.lbl.gov,verified 14 May 2009) funded by the U.S. Department of Energy, Office of Science, Office of Biological and Environmental Research, Genomics: GTL Program, through Contract DE-AC02-05CH11231 with the Lawrence Berkeley National Laboratory.
000005526 520__ $$aRecently, functional gene array-GeoChip-was introduced, targeting more than 12,000 different microbial functional genes. In addition to microbial genes involved in widespread microbial functions, this array also includes for genes involved in atrazine [6-chloro-N-ethyl-N'-(1-methylethyl)-1,3,5-triazine-2,4-diamine] degradation. study, we evaluated whether this type of microarray is suitable for screening a rural aquifer for native and their intrinsic degradation capacities. Surprisingly, small amounts of organic contaminants (up to similar to 10 mu g/L) well close to a farmyard led to the establishment of a specific degrader community. This means that although contamination at such low concentrations should have only a minor effect on water quality, the microbial community in environment reacted in a very sensitive manner to the contaminants. Specific atrazine degradation detected in the aquifer showing the highest level of contamination. Their presence was confirmed using a polymerase chain reaction approach combined with gene sequence analysis. Whether this should be an undesired ecotoxicological effect or whether it should be taken as evidence for regular natural attenuation diffuse contaminants is a question that remains to be answered by more comprehensive studies.
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000005526 7001_ $$0P:(DE-HGF)0$$aWachtmeister, T.$$b1
000005526 7001_ $$0P:(DE-HGF)0$$aZhou, J.$$b2
000005526 7001_ $$0P:(DE-Juel1)VDB202$$aBurauel, P.$$b3$$uFZJ
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000005526 8567_ $$uhttp://dx.doi.org/10.2136/vzj2008.0072
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