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@ARTICLE{Liebich:5526,
      author       = {Liebich, J. and Wachtmeister, T. and Zhou, J. and Burauel,
                      P.},
      title        = {{D}egradation of {D}iffuse {P}esticide {C}ontaminants:
                      {S}creening for {M}icrobial {P}otential {U}sing a
                      {F}unctional {G}ene {M}icroarray},
      journal      = {Vadose zone journal},
      volume       = {8},
      issn         = {1539-1663},
      address      = {Madison, Wis.},
      publisher    = {SSSA},
      reportid     = {PreJuSER-5526},
      pages        = {703 - 710},
      year         = {2009},
      note         = {We would like to thank Hans Gunther Sittardt for assistance
                      during sampling and for providing atrazine contamination
                      data, Dr. Tappe and Prof. Ruttkowski (Aachen University of
                      Applied Sciences) for helpful discussions, and Dr. Canty and
                      Hazel Rochford for their linguistic revisions. The
                      construction of the GeoChip was supported within the
                      framework of the Environmental Stress Pathway Project (ESPP)
                      of the Virtual Institute for Microbial Stress and Survival
                      (http://vimss.lbl.gov,verified 14 May 2009) funded by the
                      U.S. Department of Energy, Office of Science, Office of
                      Biological and Environmental Research, Genomics: GTL
                      Program, through Contract DE-AC02-05CH11231 with the
                      Lawrence Berkeley National Laboratory.},
      abstract     = {Recently, functional gene array-GeoChip-was introduced,
                      targeting more than 12,000 different microbial functional
                      genes. In addition to microbial genes involved in widespread
                      microbial functions, this array also includes for genes
                      involved in atrazine
                      [6-chloro-N-ethyl-N'-(1-methylethyl)-1,3,5-triazine-2,4-diamine]
                      degradation. study, we evaluated whether this type of
                      microarray is suitable for screening a rural aquifer for
                      native and their intrinsic degradation capacities.
                      Surprisingly, small amounts of organic contaminants (up to
                      similar to 10 mu g/L) well close to a farmyard led to the
                      establishment of a specific degrader community. This means
                      that although contamination at such low concentrations
                      should have only a minor effect on water quality, the
                      microbial community in environment reacted in a very
                      sensitive manner to the contaminants. Specific atrazine
                      degradation detected in the aquifer showing the highest
                      level of contamination. Their presence was confirmed using a
                      polymerase chain reaction approach combined with gene
                      sequence analysis. Whether this should be an undesired
                      ecotoxicological effect or whether it should be taken as
                      evidence for regular natural attenuation diffuse
                      contaminants is a question that remains to be answered by
                      more comprehensive studies.},
      keywords     = {J (WoSType)},
      cin          = {ICG-4},
      ddc          = {550},
      cid          = {I:(DE-Juel1)VDB793},
      pnm          = {Terrestrische Umwelt},
      pid          = {G:(DE-Juel1)FUEK407},
      shelfmark    = {Environmental Sciences / Soil Science / Water Resources},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000268871900017},
      doi          = {10.2136/vzj2008.0072},
      url          = {https://juser.fz-juelich.de/record/5526},
}