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000055895 0247_ $$2DOI$$a10.1515/BC.2007.020
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000055895 084__ $$2WoS$$aBiochemistry & Molecular Biology
000055895 1001_ $$0P:(DE-Juel1)VDB630$$aHoffmann, S.$$b0$$uFZJ
000055895 245__ $$aNef protein of human immunodeficiency virus type 1 binds its own myristoylated N-terminus
000055895 260__ $$aBerlin [u.a.]$$bde Gruyter$$c2007
000055895 300__ $$a181 - 183
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000055895 440_0 $$09042$$aBiological Chemistry$$v388$$x1431-6730
000055895 500__ $$aRecord converted from VDB: 12.11.2012
000055895 520__ $$aHIV-1 Nef is a small protein (approx. 25 kDa) that is posttranslationally modified by myristoylation. To explain its complex activities, a 'Nef-cycle' is discussed, which postulates different molecular conformations of Nef. Using recombinant full-length non-myristoylated Nef and synthetic peptides, we demonstrate by fluorescence titration experiments that a peptide representing the myristoylated N-terminus of Nef is specifically bound by Nef. A non-myristoylated N-terminal fragment of Nef or a myristoylated control peptide does not bind to Nef. These results are the first direct experimental evidence of the existence of a myristate-binding pocket in Nef, a prerequisite of the postulated 'closed' Nef conformation.
000055895 536__ $$0G:(DE-Juel1)FUEK409$$2G:(DE-HGF)$$aFunktion und Dysfunktion des Nervensystems$$cP33$$x0
000055895 588__ $$aDataset connected to Web of Science, Pubmed
000055895 650_2 $$2MeSH$$aCalorimetry: methods
000055895 650_2 $$2MeSH$$aGene Products, nef: chemistry
000055895 650_2 $$2MeSH$$aHumans
000055895 650_2 $$2MeSH$$aMyristic Acid: chemistry
000055895 650_2 $$2MeSH$$aPeptides: chemistry
000055895 650_2 $$2MeSH$$aRecombinant Proteins: chemistry
000055895 650_2 $$2MeSH$$aSpectrometry, Fluorescence
000055895 650_2 $$2MeSH$$anef Gene Products, Human Immunodeficiency Virus
000055895 650_7 $$00$$2NLM Chemicals$$aGene Products, nef
000055895 650_7 $$00$$2NLM Chemicals$$aPeptides
000055895 650_7 $$00$$2NLM Chemicals$$aRecombinant Proteins
000055895 650_7 $$00$$2NLM Chemicals$$anef Gene Products, Human Immunodeficiency Virus
000055895 650_7 $$0544-63-8$$2NLM Chemicals$$aMyristic Acid
000055895 650_7 $$2WoSType$$aJ
000055895 65320 $$2Author$$afluorescence titration
000055895 65320 $$2Author$$apeptide binding
000055895 65320 $$2Author$$aprotein conformation
000055895 7001_ $$0P:(DE-Juel1)VDB20019$$aJonas, E.$$b1$$uFZJ
000055895 7001_ $$0P:(DE-HGF)0$$aKönig, S.$$b2
000055895 7001_ $$0P:(DE-HGF)0$$aPreusser-Kunze, A.$$b3
000055895 7001_ $$0P:(DE-Juel1)132029$$aWillbold, D.$$b4$$uFZJ
000055895 773__ $$0PERI:(DE-600)1466062-3$$a10.1515/BC.2007.020$$gVol. 388, p. 181 - 183$$p181 - 183$$q388<181 - 183$$tBiological chemistry$$v388$$x1431-6730$$y2007
000055895 8567_ $$uhttp://dx.doi.org/10.1515/BC.2007.020
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