TY  - JOUR
AU  - Hoffmann, S.
AU  - Jonas, E.
AU  - König, S.
AU  - Preusser-Kunze, A.
AU  - Willbold, D.
TI  - Nef protein of human immunodeficiency virus type 1 binds its own myristoylated N-terminus
JO  - Biological chemistry
VL  - 388
SN  - 1431-6730
CY  - Berlin [u.a.]
PB  - de Gruyter
M1  - PreJuSER-55895
SP  - 181 - 183
PY  - 2007
N1  - Record converted from VDB: 12.11.2012
AB  - HIV-1 Nef is a small protein (approx. 25 kDa) that is posttranslationally modified by myristoylation. To explain its complex activities, a 'Nef-cycle' is discussed, which postulates different molecular conformations of Nef. Using recombinant full-length non-myristoylated Nef and synthetic peptides, we demonstrate by fluorescence titration experiments that a peptide representing the myristoylated N-terminus of Nef is specifically bound by Nef. A non-myristoylated N-terminal fragment of Nef or a myristoylated control peptide does not bind to Nef. These results are the first direct experimental evidence of the existence of a myristate-binding pocket in Nef, a prerequisite of the postulated 'closed' Nef conformation.
KW  - Calorimetry: methods
KW  - Gene Products, nef: chemistry
KW  - Humans
KW  - Myristic Acid: chemistry
KW  - Peptides: chemistry
KW  - Recombinant Proteins: chemistry
KW  - Spectrometry, Fluorescence
KW  - nef Gene Products, Human Immunodeficiency Virus
KW  - Gene Products, nef (NLM Chemicals)
KW  - Peptides (NLM Chemicals)
KW  - Recombinant Proteins (NLM Chemicals)
KW  - nef Gene Products, Human Immunodeficiency Virus (NLM Chemicals)
KW  - Myristic Acid (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:17261081
UR  - <Go to ISI:>//WOS:000243978600006
DO  - DOI:10.1515/BC.2007.020
UR  - https://juser.fz-juelich.de/record/55895
ER  -