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@ARTICLE{Hoffmann:55895,
      author       = {Hoffmann, S. and Jonas, E. and König, S. and
                      Preusser-Kunze, A. and Willbold, D.},
      title        = {{N}ef protein of human immunodeficiency virus type 1 binds
                      its own myristoylated {N}-terminus},
      journal      = {Biological chemistry},
      volume       = {388},
      issn         = {1431-6730},
      address      = {Berlin [u.a.]},
      publisher    = {de Gruyter},
      reportid     = {PreJuSER-55895},
      pages        = {181 - 183},
      year         = {2007},
      note         = {Record converted from VDB: 12.11.2012},
      abstract     = {HIV-1 Nef is a small protein (approx. 25 kDa) that is
                      posttranslationally modified by myristoylation. To explain
                      its complex activities, a 'Nef-cycle' is discussed, which
                      postulates different molecular conformations of Nef. Using
                      recombinant full-length non-myristoylated Nef and synthetic
                      peptides, we demonstrate by fluorescence titration
                      experiments that a peptide representing the myristoylated
                      N-terminus of Nef is specifically bound by Nef. A
                      non-myristoylated N-terminal fragment of Nef or a
                      myristoylated control peptide does not bind to Nef. These
                      results are the first direct experimental evidence of the
                      existence of a myristate-binding pocket in Nef, a
                      prerequisite of the postulated 'closed' Nef conformation.},
      keywords     = {Calorimetry: methods / Gene Products, nef: chemistry /
                      Humans / Myristic Acid: chemistry / Peptides: chemistry /
                      Recombinant Proteins: chemistry / Spectrometry, Fluorescence
                      / nef Gene Products, Human Immunodeficiency Virus / Gene
                      Products, nef (NLM Chemicals) / Peptides (NLM Chemicals) /
                      Recombinant Proteins (NLM Chemicals) / nef Gene Products,
                      Human Immunodeficiency Virus (NLM Chemicals) / Myristic Acid
                      (NLM Chemicals) / J (WoSType)},
      cin          = {INB-2},
      ddc          = {540},
      cid          = {I:(DE-Juel1)VDB805},
      pnm          = {Funktion und Dysfunktion des Nervensystems},
      pid          = {G:(DE-Juel1)FUEK409},
      shelfmark    = {Biochemistry $\&$ Molecular Biology},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:17261081},
      UT           = {WOS:000243978600006},
      doi          = {10.1515/BC.2007.020},
      url          = {https://juser.fz-juelich.de/record/55895},
}