000056130 001__ 56130 000056130 005__ 20240610120033.0 000056130 0247_ $$2pmid$$apmid:16773394 000056130 0247_ $$2DOI$$a10.1007/s00249-006-0076-4 000056130 0247_ $$2WOS$$aWOS:000240899300005 000056130 037__ $$aPreJuSER-56130 000056130 041__ $$aeng 000056130 082__ $$a570 000056130 084__ $$2WoS$$aBiophysics 000056130 1001_ $$0P:(DE-HGF)0$$aPapadopoulos, G.$$b0 000056130 245__ $$aChanges in the level of poly(Phe) synthesis in Escherichia coli ribosomes containing mutants of L4 ribosomal protein from Thermus thermophilus can be explained by structural changes in the peptidyltransferase center: a molecular dynamics simulation anal. 000056130 260__ $$aBerlin$$bSpringer$$c2006 000056130 300__ $$a675 - 683 000056130 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article 000056130 3367_ $$2DataCite$$aOutput Types/Journal article 000056130 3367_ $$00$$2EndNote$$aJournal Article 000056130 3367_ $$2BibTeX$$aARTICLE 000056130 3367_ $$2ORCID$$aJOURNAL_ARTICLE 000056130 3367_ $$2DRIVER$$aarticle 000056130 440_0 $$010441$$aEuropean Biophysics Journal : with Biophysics Letters$$v35$$x0175-7571$$y8 000056130 500__ $$aRecord converted from VDB: 12.11.2012 000056130 520__ $$aData from polyphenylalanine [poly(Phe)] synthesis determination in the presence and in the absence of erythromycin have been used in conjunction with Molecular Dynamics Simulation analysis, in order to localize the functional sites affected by mutations of Thermus thermophilus ribosomal protein L4 incorporated in Escherichia coli ribosomes. We observed that alterations in ribosome capability to synthesize poly(Phe) in the absence of erythromycin were mainly correlated to shifts of A2062 and C2612 of 23S rRNA, while in the presence of erythromycin they were correlated to shifts of A2060 and U2584 of 23S rRNA. Our results suggest a means of understanding the role of the extended loop of L4 ribosomal protein in ribosomal peptidyltransferase center. 000056130 536__ $$0G:(DE-Juel1)FUEK414$$2G:(DE-HGF)$$aKondensierte Materie$$cP54$$x0 000056130 588__ $$aDataset connected to Web of Science, Pubmed 000056130 650_2 $$2MeSH$$aAmino Acid Sequence 000056130 650_2 $$2MeSH$$aAnti-Bacterial Agents: pharmacology 000056130 650_2 $$2MeSH$$aErythromycin: pharmacology 000056130 650_2 $$2MeSH$$aEscherichia coli: drug effects 000056130 650_2 $$2MeSH$$aEscherichia coli: genetics 000056130 650_2 $$2MeSH$$aEscherichia coli: metabolism 000056130 650_2 $$2MeSH$$aModels, Molecular 000056130 650_2 $$2MeSH$$aMolecular Sequence Data 000056130 650_2 $$2MeSH$$aMutation 000056130 650_2 $$2MeSH$$aNucleic Acid Conformation 000056130 650_2 $$2MeSH$$aPeptides: metabolism 000056130 650_2 $$2MeSH$$aPeptidyl Transferases: chemistry 000056130 650_2 $$2MeSH$$aPeptidyl Transferases: metabolism 000056130 650_2 $$2MeSH$$aProtein Conformation 000056130 650_2 $$2MeSH$$aRNA, Bacterial: genetics 000056130 650_2 $$2MeSH$$aRNA, Ribosomal, 23S: genetics 000056130 650_2 $$2MeSH$$aRibosomal Proteins: chemistry 000056130 650_2 $$2MeSH$$aRibosomal Proteins: genetics 000056130 650_2 $$2MeSH$$aRibosomal Proteins: metabolism 000056130 650_2 $$2MeSH$$aRibosomes: genetics 000056130 650_2 $$2MeSH$$aRibosomes: metabolism 000056130 650_2 $$2MeSH$$aThermus thermophilus: metabolism 000056130 650_7 $$00$$2NLM Chemicals$$aAnti-Bacterial Agents 000056130 650_7 $$00$$2NLM Chemicals$$aPeptides 000056130 650_7 $$00$$2NLM Chemicals$$aRNA, Bacterial 000056130 650_7 $$00$$2NLM Chemicals$$aRNA, Ribosomal, 23S 000056130 650_7 $$00$$2NLM Chemicals$$aRibosomal Proteins 000056130 650_7 $$00$$2NLM Chemicals$$aribosomal protein L4 000056130 650_7 $$0114-07-8$$2NLM Chemicals$$aErythromycin 000056130 650_7 $$030394-07-1$$2NLM Chemicals$$apolyphenylalanine 000056130 650_7 $$0EC 2.3.2.12$$2NLM Chemicals$$aPeptidyl Transferases 000056130 650_7 $$2WoSType$$aJ 000056130 65320 $$2Author$$amolecular dynamics 000056130 65320 $$2Author$$aribosomal function 000056130 65320 $$2Author$$aerythromycin 000056130 7001_ $$0P:(DE-Juel1)VDB10417$$aGrudinin, S.$$b1$$uFZJ 000056130 7001_ $$0P:(DE-HGF)0$$aKalpaxis, D.$$b2 000056130 7001_ $$0P:(DE-HGF)0$$aCholi-Papadopoulou, T.$$b3 000056130 773__ $$0PERI:(DE-600)1398349-0$$a10.1007/s00249-006-0076-4$$gVol. 35, p. 675 - 683$$p675 - 683$$q35<675 - 683$$tEuropean biophysics journal$$v35$$x0175-7571$$y2006 000056130 8567_ $$uhttp://dx.doi.org/10.1007/s00249-006-0076-4 000056130 909CO $$ooai:juser.fz-juelich.de:56130$$pVDB 000056130 9131_ $$0G:(DE-Juel1)FUEK414$$bMaterie$$kP54$$lKondensierte Materie$$vKondensierte Materie$$x0$$zentfällt bis 2009 000056130 9141_ $$aNachtrag$$y2006 000056130 915__ $$0StatID:(DE-HGF)0010$$aJCR/ISI refereed 000056130 9201_ $$0I:(DE-Juel1)VDB31$$d31.12.2006$$gIFF$$kIFF-TH-II$$lTheorie II$$x1 000056130 970__ $$aVDB:(DE-Juel1)87881 000056130 980__ $$aVDB 000056130 980__ $$aConvertedRecord 000056130 980__ $$ajournal 000056130 980__ $$aI:(DE-Juel1)ICS-2-20110106 000056130 980__ $$aUNRESTRICTED 000056130 981__ $$aI:(DE-Juel1)IBI-5-20200312 000056130 981__ $$aI:(DE-Juel1)IAS-2-20090406 000056130 981__ $$aI:(DE-Juel1)ICS-2-20110106