TY  - JOUR
AU  - Papadopoulos, G.
AU  - Grudinin, S.
AU  - Kalpaxis, D.
AU  - Choli-Papadopoulou, T.
TI  - Changes in the level of poly(Phe) synthesis in Escherichia coli ribosomes containing mutants of L4 ribosomal protein from Thermus thermophilus can be explained by structural changes in the peptidyltransferase center: a molecular dynamics simulation anal.
JO  - European biophysics journal
VL  - 35
SN  - 0175-7571
CY  - Berlin
PB  - Springer
M1  - PreJuSER-56130
SP  - 675 - 683
PY  - 2006
N1  - Record converted from VDB: 12.11.2012
AB  - Data from polyphenylalanine [poly(Phe)] synthesis determination in the presence and in the absence of erythromycin have been used in conjunction with Molecular Dynamics Simulation analysis, in order to localize the functional sites affected by mutations of Thermus thermophilus ribosomal protein L4 incorporated in Escherichia coli ribosomes. We observed that alterations in ribosome capability to synthesize poly(Phe) in the absence of erythromycin were mainly correlated to shifts of A2062 and C2612 of 23S rRNA, while in the presence of erythromycin they were correlated to shifts of A2060 and U2584 of 23S rRNA. Our results suggest a means of understanding the role of the extended loop of L4 ribosomal protein in ribosomal peptidyltransferase center.
KW  - Amino Acid Sequence
KW  - Anti-Bacterial Agents: pharmacology
KW  - Erythromycin: pharmacology
KW  - Escherichia coli: drug effects
KW  - Escherichia coli: genetics
KW  - Escherichia coli: metabolism
KW  - Models, Molecular
KW  - Molecular Sequence Data
KW  - Mutation
KW  - Nucleic Acid Conformation
KW  - Peptides: metabolism
KW  - Peptidyl Transferases: chemistry
KW  - Peptidyl Transferases: metabolism
KW  - Protein Conformation
KW  - RNA, Bacterial: genetics
KW  - RNA, Ribosomal, 23S: genetics
KW  - Ribosomal Proteins: chemistry
KW  - Ribosomal Proteins: genetics
KW  - Ribosomal Proteins: metabolism
KW  - Ribosomes: genetics
KW  - Ribosomes: metabolism
KW  - Thermus thermophilus: metabolism
KW  - Anti-Bacterial Agents (NLM Chemicals)
KW  - Peptides (NLM Chemicals)
KW  - RNA, Bacterial (NLM Chemicals)
KW  - RNA, Ribosomal, 23S (NLM Chemicals)
KW  - Ribosomal Proteins (NLM Chemicals)
KW  - ribosomal protein L4 (NLM Chemicals)
KW  - Erythromycin (NLM Chemicals)
KW  - polyphenylalanine (NLM Chemicals)
KW  - Peptidyl Transferases (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:16773394
UR  - <Go to ISI:>//WOS:000240899300005
DO  - DOI:10.1007/s00249-006-0076-4
UR  - https://juser.fz-juelich.de/record/56130
ER  -