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000056151 0247_ $$2DOI$$a10.1002/bit.21166
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000056151 084__ $$2WoS$$aBiotechnology & Applied Microbiology
000056151 1001_ $$0P:(DE-Juel1)VDB38056$$aFrerix, A.$$b0$$uFZJ
000056151 245__ $$aSeparation of Genomic DNA, RNA, and Open Circular Plasmid DNA From Supercoiled Plasmid DNA by Combining Denaturatin, Selective Renaturation and Aqueous Two-Phase Extraction
000056151 260__ $$aNew York, NY [u.a.]$$bWiley$$c2007
000056151 300__ $$a57 - 66
000056151 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article
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000056151 440_0 $$0900$$aBiotechnology and Bioengineering$$v96$$x0006-3592$$y1
000056151 500__ $$aRecord converted from VDB: 12.11.2012
000056151 520__ $$aIn the current study we developed a process for the capture of pDNA exploiting the ability of aqueous two-phase systems to differentiate between different forms of DNA. In these systems scpDNA exhibits a near quantitative partitioning in the salt-rich bottom phase. The successive recovery from the salt rich bottom phase is accomplished by a novel membrane step. The polish operation to meet final purity demands is again based on a system exploiting a combination of the denaturation of the nucleic acids present, specific renaturation of scpDNA, and an ATP system able to differentiate between the renatured scpDNA and the denatured contaminants such as ocpDNA and genomic host DNA. This polish step thus allows a rapid and efficient separation of scpDNA from contaminating nucleic acids which up to date otherwise only can be accomplished with much more cumbersome chromatographic methods. In a benchmark comparison, it could be shown that the newly developed process exhibits a comparable yield to an industrial standard process while at the same time showing superior performance in terms of purity and process time. Additionally it could be shown that the developed polish procedure can be applied as a standalone module to support already existing processes.
000056151 536__ $$0G:(DE-Juel1)FUEK410$$2G:(DE-HGF)$$aBiotechnologie$$cPBT$$x0
000056151 588__ $$aDataset connected to Web of Science, Pubmed
000056151 650_2 $$2MeSH$$aChemical Fractionation: methods
000056151 650_2 $$2MeSH$$aDNA: genetics
000056151 650_2 $$2MeSH$$aDNA: isolation & purification
000056151 650_2 $$2MeSH$$aDNA, Circular: genetics
000056151 650_2 $$2MeSH$$aDNA, Circular: isolation & purification
000056151 650_2 $$2MeSH$$aElectrophoresis, Agar Gel: methods
000056151 650_2 $$2MeSH$$aPhase Transition
000056151 650_2 $$2MeSH$$aPlasmids: genetics
000056151 650_2 $$2MeSH$$aPlasmids: isolation & purification
000056151 650_2 $$2MeSH$$aRNA: genetics
000056151 650_2 $$2MeSH$$aRNA: isolation & purification
000056151 650_2 $$2MeSH$$aUltrafiltration: methods
000056151 650_2 $$2MeSH$$aWater: chemistry
000056151 650_7 $$00$$2NLM Chemicals$$aDNA, Circular
000056151 650_7 $$063231-63-0$$2NLM Chemicals$$aRNA
000056151 650_7 $$07732-18-5$$2NLM Chemicals$$aWater
000056151 650_7 $$09007-49-2$$2NLM Chemicals$$aDNA
000056151 650_7 $$2WoSType$$aJ
000056151 65320 $$2Author$$aaqueous two-phase system (ATPS)
000056151 65320 $$2Author$$aplasmid DNA (pDNA)
000056151 65320 $$2Author$$adenaturation
000056151 65320 $$2Author$$asupercoiled plasmid DNA (scpDNA)
000056151 65320 $$2Author$$aopen circular plasmid DNA (ocpDNA)
000056151 65320 $$2Author$$agenomic DNA (gDNA)
000056151 7001_ $$0P:(DE-Juel1)VDB61421$$aGeilenkirchen, P.$$b1$$uFZJ
000056151 7001_ $$0P:(DE-HGF)0$$aMüller, M.$$b2
000056151 7001_ $$0P:(DE-HGF)0$$aKula, M.-R.$$b3
000056151 7001_ $$0P:(DE-Juel1)VDB21816$$aHubbuch, J.$$b4$$uFZJ
000056151 773__ $$0PERI:(DE-600)1480809-2$$a10.1002/bit.21166$$gVol. 96, p. 57 - 66$$p57 - 66$$q96<57 - 66$$tBiotechnology & bioengineering$$v96$$x0006-3592$$y2007
000056151 8567_ $$uhttp://dx.doi.org/10.1002/bit.21166
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000056151 915__ $$0StatID:(DE-HGF)0010$$aJCR/ISI refereed
000056151 9201_ $$0I:(DE-Juel1)VDB56$$gIBT$$kIBT-2$$lBiotechnologie 2$$x0$$zab 31.10.10 weitergeführt IBG-1
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