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@ARTICLE{Frerix:56151,
      author       = {Frerix, A. and Geilenkirchen, P. and Müller, M. and Kula,
                      M.-R. and Hubbuch, J.},
      title        = {{S}eparation of {G}enomic {DNA}, {RNA}, and {O}pen
                      {C}ircular {P}lasmid {DNA} {F}rom {S}upercoiled {P}lasmid
                      {DNA} by {C}ombining {D}enaturatin, {S}elective
                      {R}enaturation and {A}queous {T}wo-{P}hase {E}xtraction},
      journal      = {Biotechnology $\&$ bioengineering},
      volume       = {96},
      issn         = {0006-3592},
      address      = {New York, NY [u.a.]},
      publisher    = {Wiley},
      reportid     = {PreJuSER-56151},
      pages        = {57 - 66},
      year         = {2007},
      note         = {Record converted from VDB: 12.11.2012},
      abstract     = {In the current study we developed a process for the capture
                      of pDNA exploiting the ability of aqueous two-phase systems
                      to differentiate between different forms of DNA. In these
                      systems scpDNA exhibits a near quantitative partitioning in
                      the salt-rich bottom phase. The successive recovery from the
                      salt rich bottom phase is accomplished by a novel membrane
                      step. The polish operation to meet final purity demands is
                      again based on a system exploiting a combination of the
                      denaturation of the nucleic acids present, specific
                      renaturation of scpDNA, and an ATP system able to
                      differentiate between the renatured scpDNA and the denatured
                      contaminants such as ocpDNA and genomic host DNA. This
                      polish step thus allows a rapid and efficient separation of
                      scpDNA from contaminating nucleic acids which up to date
                      otherwise only can be accomplished with much more cumbersome
                      chromatographic methods. In a benchmark comparison, it could
                      be shown that the newly developed process exhibits a
                      comparable yield to an industrial standard process while at
                      the same time showing superior performance in terms of
                      purity and process time. Additionally it could be shown that
                      the developed polish procedure can be applied as a
                      standalone module to support already existing processes.},
      keywords     = {Chemical Fractionation: methods / DNA: genetics / DNA:
                      isolation $\&$ purification / DNA, Circular: genetics / DNA,
                      Circular: isolation $\&$ purification / Electrophoresis,
                      Agar Gel: methods / Phase Transition / Plasmids: genetics /
                      Plasmids: isolation $\&$ purification / RNA: genetics / RNA:
                      isolation $\&$ purification / Ultrafiltration: methods /
                      Water: chemistry / DNA, Circular (NLM Chemicals) / RNA (NLM
                      Chemicals) / Water (NLM Chemicals) / DNA (NLM Chemicals) / J
                      (WoSType)},
      cin          = {IBT-2},
      ddc          = {570},
      cid          = {I:(DE-Juel1)VDB56},
      pnm          = {Biotechnologie},
      pid          = {G:(DE-Juel1)FUEK410},
      shelfmark    = {Biotechnology $\&$ Applied Microbiology},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:16937402},
      UT           = {WOS:000243176500007},
      doi          = {10.1002/bit.21166},
      url          = {https://juser.fz-juelich.de/record/56151},
}